Onion juice and extracts for the inhibition of enzymatic browning mechanisms in frozen Agaricus bisporus mushrooms

J Sci Food Agric. 2021 Aug 15;101(10):4099-4107. doi: 10.1002/jsfa.11045. Epub 2021 Jan 8.

Abstract

Background: The potential of onion juice, as well as extracts of waste (tunic) (5%) and fleshy scale leaves (25%), to inhibit enzymatic browning of frozen Agaricus bisporus was investigated. The onion materials were used for blanching and their effectiveness in conserving integrity and appearance of mushroom fruiting bodies was compared with the currently accepted method of blanching in a sodium metabisulfite (SM) solution.

Results: It was observed that l-phenylalanine content may be a useful indicator of the changes in enzymatic activity during frozen storage, and l-tyrosine may be an indicator of a loss of lightness in color (parameter L*). The enzymes responsible for color changes were mainly monophenolase (MON) and, to a lesser degree, diphenolase (DIP). After being stored frozen for 8 months, these enzymes were detected at a 29:1 (DIP:MON) ratio in untreated mushrooms and a 2:1 (DIP:MON) ratio in mushrooms treated with onion juice.

Conclusion: Onion products may be a good alternative to an SM solution. The most effective method to conserve the light color of fruiting bodies was blanching in juice or in an extract of the fleshy scale leaves. The least effective inhibitor of MON was tunic extract, which did, however, cause a favourable increase in the reducing capacity (total polyphenols) and flavonoids. Although the onion waste (tunic) extract changed the color of mushrooms from white to creamy orange, the color of these products was attractive and positively evaluated by panellists. © 2020 Society of Chemical Industry.

Keywords: blanching; browning inhibitors; button mushrooms; freezing; onion.

Publication types

  • Evaluation Study

MeSH terms

  • Agaricus / chemistry
  • Agaricus / drug effects
  • Agaricus / enzymology*
  • Color
  • Food Preservation / methods*
  • Food Preservatives / pharmacology*
  • Fungal Proteins / chemistry
  • Fungal Proteins / metabolism*
  • Onions / chemistry*
  • Plant Extracts / pharmacology*
  • Sulfites / pharmacology

Substances

  • Food Preservatives
  • Fungal Proteins
  • Plant Extracts
  • Sulfites
  • sodium metabisulfite

Supplementary concepts

  • Agaricus bisporus