Abstract
The effect of HDACs 4 and 5 on the level of atrophy, calpain-1 and titin content, and TTN gene expression in rat soleus after 7-day gravitational unloading (hindlimb suspension model) was studied. The development of atrophic changes induced by gravitational unloading in rat soleus was accompanied by an increase in the calpain-1 content, an increase in titin proteolysis, and a decrease in the mRNA content of the protein. Inhibition of HDACs 4 and 5 did not eliminate the development of unloading-induced atrophy but significantly prevented proteolysis of titin and the decrease in the TTN gene expression.
Keywords:
TTN; s: gravitational unloading; skeletal muscle; titin.
MeSH terms
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Animals
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Benzamides / pharmacology*
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Calpain / metabolism
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Connectin / genetics
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Connectin / metabolism*
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Disease Models, Animal
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Gene Expression / drug effects
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Hindlimb Suspension / methods
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Histone Deacetylase Inhibitors / pharmacology*
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Histone Deacetylases / chemistry
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Histone Deacetylases / metabolism*
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Male
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Muscle, Skeletal / drug effects*
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Muscle, Skeletal / metabolism
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Muscle, Skeletal / pathology
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Muscular Atrophy / drug therapy*
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Muscular Atrophy / genetics
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Muscular Atrophy / metabolism
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Muscular Atrophy / pathology
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Proteolysis / drug effects
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Rats
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Rats, Wistar
Substances
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Benzamides
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Connectin
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Histone Deacetylase Inhibitors
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LMK-235
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Ttn protein, rat
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Calpain
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Capn1 protein, rat
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HDAC4 protein, rat
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Hdac5 protein, rat
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Histone Deacetylases