The complete chloroplast genome of an endangered orchid Paphiopedilum spicerianum

Li-Ping Ge; Lu Tang; Lu Li; Yan Luo

doi:10.1080/23802359.2020.1830727

The complete chloroplast genome of an endangered orchid Paphiopedilum spicerianum

Mitochondrial DNA B Resour. 2020 Oct 21;5(3):3612-3613. doi: 10.1080/23802359.2020.1830727.

Authors

Li-Ping Ge¹, Lu Tang^{1

2}, Lu Li³, Yan Luo^{2

4}

Affiliations

¹ College of Forestry, Shanxi Agricultural University, Jinzhong, China.
² Gardening and Horticulture Department, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla, China.
³ Department of Biodiversity Conservation, Yunnan Academy of Biodiversity, Southwest Forestry University, Kunming, China.
⁴ Gardening and Horticulture Department, Core Botanical Gardens, Chinese Academy of Sciences, Mengla, China.

Abstract

Paphiopedilum spicerianum (H. G. Reichenbach) Pfitzer is an endangered and threatened orchid species in China. The genetic and molecular data of this orchid species is deficient. With the aim to identify appropriate chloroplast markers for the use in a conservation biology study, the complete chloroplast genome of P. spicerianum was reported. We found that the chloroplast genome of P. spicerianum is 157,292 bp in length, containing a large single-copy region of 87,252 bp, a small single-copy region of 1828 bp, and a pair of inverted repeats of 34,106 bp. Genome annotation predicted 105 unique genes, including 71 protein-coding genes, 30 tRNAs, and four rRNAs. Fourteen genes contained one intron and two genes (clpP and ycf3) had two introns. The GC content of the P. spicerianum was 35.8%. Phylogenetic analysis indicated P. spicerianum was closely related to P. purpuratum.

Keywords: Orchidaceae; Paphiopedilum spicerianum; chloroplast genome; phylogenomics.

Grants and funding

This work was supported by the National Natural Science Foundation of China [Grant No. 31870183], the Natural Science Foundation of Shanxi Province [201901D111223], and Southeast Asia Biodiversity Research Institute, Chinese Academy of Sciences [Y4ZK111B01].