Characterization of complete mitochondrial genome of Hylarana guentheri (Anura: Ranidae) and its phylogenetic implication

Lichun Jiang; Bingxiu Wu; Jing Luo; Zhongwen Xu; Nenghao Huang

doi:10.1080/23802359.2019.1711216

Characterization of complete mitochondrial genome of Hylarana guentheri (Anura: Ranidae) and its phylogenetic implication

Mitochondrial DNA B Resour. 2020 Jan 14;5(1):616-618. doi: 10.1080/23802359.2019.1711216.

Authors

Lichun Jiang^{1

2}, Bingxiu Wu¹, Jing Luo¹, Zhongwen Xu¹, Nenghao Huang¹

Affiliations

¹ Key Laboratory for Molecular Biology and Biopharmaceutics, School of Life Science and Technology, Mianyang Normal University, Mianyang, P.R. China.
² Ecological Security and Protection Key Laboratory of Sichuan Province, Mianyang Normal University, Mianyang, P.R. China.

Abstract

The Günther's frog (Hylarana guentheri) belongs to a member of the family Ranidae. We provide a complete mitogenome of H. guentheri and examine its phylogenetic position with other related species. Its mitogenome is a closed circular molecule 18,698 bp in length including 13 protein coding genes, 22 tRNA coding genes, two rRNA-coding genes, and a control region (CR) that are conserved in most Ranidae mitogenomes. The overall base composition of the H. guentheri mitogenome is 29.27% A, 30.45% T, 26.14% C, and 14.15% G, which is typical for Amphibious animals' mitochondrial genomes. The alignment of the Ranidae species control regions showed high levels of genetic variation and abundant AT content. Seven tandem repeats were found in the control region. Phylogenetic analysis with Bayesian inference and maximum likelihood based on 13 protein-coding genes indicated that H. guentheri is more closely related to Nidirana okinavana than to Babina subaspera and B. holsti. The complete mitogenome of H. guentheri provides a potentially useful resource for further exploration of the taxonomic status and phylogenetic relationships of Hylarana and related species.

Keywords: Hylarana guentheri; genome organization; mitochondrial genome; phylogenetic status; ranidae.

Grants and funding

This research was supported by the Research Project of Ecological Security and Protection Key Laboratory of Sichuan Province [No. ESP1706], the Research Project of Education Office Project of Sichuan Province [No. 18ZA0261], Scientific Research Foundation Projects of Mianyang Teacher’s College [No. QD2015A003], the Scientific Research Fund of Mianyang Teacher’s College [No.PY-2016-A03, MYSY2017JC02 and Mnu-JY18285].