Chloroplast characterization and phylogenetic relationship of Cymbidium aloifolium (Orchidaceae)

Juan Chen; Ming-Kun Chen; Qing-Dong Zheng; Shan-Hu Ma; Zhong-Jian Liu; Ye Ai

doi:10.1080/23802359.2019.1704656

Chloroplast characterization and phylogenetic relationship of Cymbidium aloifolium (Orchidaceae)

Mitochondrial DNA B Resour. 2020 Jan 10;5(1):478-479. doi: 10.1080/23802359.2019.1704656.

Authors

Juan Chen¹, Ming-Kun Chen¹, Qing-Dong Zheng¹, Shan-Hu Ma¹, Zhong-Jian Liu¹, Ye Ai¹

Affiliation

¹ Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, China.

Abstract

Cymbidium aloifolium is an epiphytic orchid with high medicinal and ornamental value. In order to get a deeper understanding of C. aloifolium, we determined the complete chloroplast genome of C. aloifolium by Illumina sequencing data. The length of this genome is 157,328 bp, including a couple of inverted repeat (IR) regions of 26,829 bp, a large single-copy (LSC) region of 85,793 bp, and a small single-copy (SSC) region of 17,877 bp. The chloroplast genome comprised of 139 genes, including 78 protein-coding genes, 38 tRNA genes, and 8 rRNA genes. In addition, the phylogenetic analysis based on 17 chloroplast genomes of Orchidaceae indicated that C. mannii was closely related to C. aloifolium. This study will provide more valuable information for the classification and phylogenetic research of Cymbidium genus.

Keywords: Cymbidium aloifolium; Illumina sequencing; Orchid; chloroplast genome.

Grants and funding

This work was supported by the National Natural Science Foundation of China [No. 31700618] and the Innovation Project of Fujian Agriculture and Forestry University [No. KFA17331A].