Complete mitochondrial genome of the important bio-control fungus Purpureocillium lilacinum (Ophiocordycipitaceae, Hypocreales) and its phylogenetic analysis

Jianping Li; Guodong Zhang; Hong Yu; Luodong Huang; Wenbo Zeng; Yuanbing Wang

doi:10.1080/23802359.2019.1699466

Complete mitochondrial genome of the important bio-control fungus Purpureocillium lilacinum (Ophiocordycipitaceae, Hypocreales) and its phylogenetic analysis

Mitochondrial DNA B Resour. 2019 Dec 13;5(1):240-242. doi: 10.1080/23802359.2019.1699466.

Authors

Jianping Li¹, Guodong Zhang^{2

3

4}, Hong Yu^{2

3}, Luodong Huang⁵, Wenbo Zeng⁶, Yuanbing Wang^{2

3

4}

Affiliations

¹ Sanqi College, Wenshan University, Wenshan, China.
² Yunnan Herbal Laboratory, School of Life Sciences, Yunnan University, Kunming, China.
³ The International Joint Research Center for Sustainable Utilization of Cordyceps Bioresources in China and Southeast Asia, Yunnan University, Kunming, China.
⁴ The Research Center of Cordyceps Development and Utilization of Kunming, Yunnan Herbal Biotech Co. Ltd, Kunming, China.
⁵ College of Life Science and Tecnology, Guangxi University, Nanning, China.
⁶ Wenshan Biological Resources Development and Research Center, Wenshan University, Wenshan, China.

Abstract

Purpureocillium lilacinum is widely used as commercialized bio-control agents for controlling plant parasitic nematodes, as well as other insects and plant pathogens. In this study, the complete mitogenome of P. lilacinum was determined using the next-generation sequencing technology. The mitogenome is a circular molecule of 23,495 bp containing 15 protein-coding genes (PCGs), 2 rRNA (rnl and rns) genes and 22 tRNA genes. The overall base composition is 35.5% A, 36.0% T, 12.9% C and 15.6% G, with a CG content of 28.5%. Phylogenetic analysis inferred from 14 concatenated PCGs of 47 taxa shows that P. lilacinum is clustered with the genus Tolypocladium of Ophiocordycipitaceae and forms a separate clade with strong statistical support. This study contributes to our understanding about sytematics and evolutionary biology of cordycipitoid fungi.

Keywords: Mitochondrial genome; Purpureocillium lilacinum; phylogenetic analysis.

Grants and funding

This work was jointly funded by the China Postdoctoral Science Foundation [2017M613017], and the National Natural Science Foundation of China [31760011, 31870017], and the Department of Science and Technology of Yunnan Province [2018IA075, 2018FY001(-006)], and the Biodiversity Survey and Assessment Project of the Ministry of Ecology and Environment, China [2019HJ2096001006].