The complete chloroplast genome sequence of the threatened Cypripedium calceolus (Orchidaceae)

Li-Jie Zhang; Rui Ding; Wei-Wei Meng; Hua-Lei Hu; Xu-Hui Chen; Hai-Hong Wu

doi:10.1080/23802359.2019.1693933

The complete chloroplast genome sequence of the threatened Cypripedium calceolus (Orchidaceae)

Mitochondrial DNA B Resour. 2019 Nov 22;4(2):4220-4222. doi: 10.1080/23802359.2019.1693933.

Authors

Li-Jie Zhang¹, Rui Ding², Wei-Wei Meng³, Hua-Lei Hu³, Xu-Hui Chen³, Hai-Hong Wu⁴

Affiliations

¹ College of Forestry, Shenyang Agricultural University, Shenyang, China.
² College of Land and Environment, Shenyang Agricultural University, Shenyang, China.
³ College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, China.
⁴ Institute of Floriculture, Liaoning Academy of Agricultural Sciences, Shenyang, China.

Abstract

The complete chloroplast genome of Cypripedium calceolus, a rare species in the family Orchidaceae was reported in this study. The genome size is 175,122 bp in length, and contains four sub-regions: 97,486 bp of large single copy (LSC) and 22,260 bp of small copy (SSC) regions, separated by 27,688 bp of inverted repeat (IR) regions. A total of 133 genes were annotated, including 87 protein-coding genes, 38 tRNA genes and 8 rRNA genes. The GC content of this cp genome is 34.36%. Phylogenetic analysis revealed a close relationship between C. calceolus with C. japonicum and C. formosanum. This is the first complete cp genome for C. calceolus that would be useful for conservation and phylogenetic studies of this species.

Keywords: Chloroplast genome; Cypripedium calceolus; conservation; rare orchid.