Complete chloroplast genome sequence of Pueraria thomsonii, an important traditional Chinese medicine plant

Xiao-Rong Miao; Jun-Qi Niu; Ai-Qin Wang; Dao-Bo Wang; Jing Fan

doi:10.1080/23802359.2019.1693301

Complete chloroplast genome sequence of Pueraria thomsonii, an important traditional Chinese medicine plant

Mitochondrial DNA B Resour. 2019 Nov 22;4(2):4163-4165. doi: 10.1080/23802359.2019.1693301.

Authors

Xiao-Rong Miao^{1

2}, Jun-Qi Niu^{1

2}, Ai-Qin Wang^{1

2}, Dao-Bo Wang^{1

2}, Jing Fan³

Affiliations

¹ College of Biology and Pharmacy, Yulin Normal University, Yulin, China.
² Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology, Yulin, China.
³ College of Life Sciences, Leshan Normal University, Leshan, China.

Abstract

Pueraria thomsonii is a leguminous plant with high root yield and starch content. It is also a medicinal material in the Chinese pharmacopeia. However, the raw materials of P. thomsonii are often confused with some non-medicinal Pueraria plants. To enrich the genetic resources of P. thomsonii and guide its molecular identification, the complete chloroplast genome was sequenced and reported. The total genome of P. thomsonii is 153,434 bp in length. consisting of two inverted repeat regions (IR_S, 25,640 bp each) separated by a large single-copy (LSC, 84,155 bp) and a small single-copy region (SSC, 17,999 bp). The overall GC content is 35.41%. It contains 130 genes, including 85 protein coding genes, 8 rRNA genes and 37 tRNA genes. Phylogenetic analysis showed that P. thomsonii could be distinguished from other plants and closely related to the legume Pachyrhizus erosus. This study enriches the genetic information of P. thomsonii and contributes to the screening of excellent germplasm.

Keywords: Pueraria thomsonii; complete chloroplast genome; illumina sequencing; legume.