The complete chloroplast genome sequence of Paphiopedilum purpuratum (Orchidaceae)

Xian-De Chen; Dong-Hui Peng; Si-Ren Lan; Jun Chen; Wen-Qun Fu

doi:10.1080/23802359.2019.1688106

The complete chloroplast genome sequence of Paphiopedilum purpuratum (Orchidaceae)

Mitochondrial DNA B Resour. 2019 Nov 8;4(2):3910-3911. doi: 10.1080/23802359.2019.1688106.

Authors

Xian-De Chen^{1

2

3}, Dong-Hui Peng^{1

2}, Si-Ren Lan^{1

2}, Jun Chen³, Wen-Qun Fu³

Affiliations

¹ Fujian Ornamental Plant Germplasm Resources Innovation and Engineering Application Research Center, Fuzhou, China.
² Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, China.
³ College of Biological Science and Biotechnology, Minnan Normal University, Zhangzhou, China.

Abstract

Paphiopedilum purpuratum, an endangered terrestrial orchid distributed in southwestern and south of China. In this study, the complete chloroplast genome (cpDNA) sequence of P. purpuratum was determined from Illumina pair-end sequencing data. With a total length of 158,459 bp in length and includes two inverted repeat regions (IRs) of 34,484 bp each, which were separated by a large single-copy region (LSC) 88,022 bp and a small single-copy region (SSC) 1,469 bp. The chloroplast genome contained 126 genes, including 74 protein conding genes,38 tRNA genes, and 8 rRNA genes. Phylogenetic analysis indicated that P. purpuratum, P. dianthum, P. niveum, P. delenatii, and P. armeniacum cluster together, placed them within genus Paphiopedilum. The complete chloroplast genome sequence of P. purpuratum will provide a useful resource for the evolutionary biology study of phylogenetic studies in Orchidaceae.

Keywords: Chloroplast genome; Illumina sequencing; Paphiopedilum purpuratum; phylogenetic.