Characterisation of the complete mitochondrial genome of Lucanus chengyuani (Coleoptera: Lucanidae)

Liang-Jong Wang; Jen-Pan Huang; Shiuh-Feng Shiao; Hsin-Ping Ko; Chia-Hsuan Sung

doi:10.1080/23802359.2019.1674703

Characterisation of the complete mitochondrial genome of Lucanus chengyuani (Coleoptera: Lucanidae)

Mitochondrial DNA B Resour. 2019 Oct 12;4(2):3460-3462. doi: 10.1080/23802359.2019.1674703.

Authors

Liang-Jong Wang¹, Jen-Pan Huang², Shiuh-Feng Shiao³, Hsin-Ping Ko⁴, Chia-Hsuan Sung⁵

Affiliations

¹ Division of Forest Protection, Taiwan Forestry Research Institute, Taipei, Taiwan.
² Biodiversity Research Center, Academia Sinica, Taipei, Taiwan.
³ Department of Entomology, National Taiwan University, Taipei, Taiwan.
⁴ Taiwan Insect Museum, Taipei, Taiwan.
⁵ Planning and Information Division, Fisheries Research Institute, Keelung, Taiwan.

Abstract

We sequenced and assembled the complete mitochondrial genome of Lucanus chengyuani, from the Alishan, Chiayi County, Taiwan. The length of the complete mitogenome of L. chengyuani is 16,926 bp and the mitogenome contains 13 protein-coding, 22 tRNA, and 2 rDNA genes. Nucleotide compositions of the whole mitogenome of L. chengyuani are 38.37% for A, 27.96% for T, 23.03% for C, and 10.637% for G. The AT and GC skewness of mitogenome sequence are 0.157 and -0.368, showing the A-skew and C-skew. The reconstructed phylogenetic relationships of 9 Lucanidae species based on 13 mitochondrial protein-coding genes are highly supported. The clade including Neolucanus maximus and Odontolabis cuvera is sister to the rest of the stag beetle clades, which contains L. chengyuani and L. mazama. Mitogenomic data from this study will provide useful information for further studies for the population genetics, speciation, biogeography, and conservation of L. chengyuani in the future.

Keywords: Lucanidae; Lucanus chengyuani; Mitochondrial genome; next-generation sequencing.