Next-generation sequencing yields the complete chloroplast genome of Pleione forrestii (Orchidaceae)

Sha-Sha Wu; Xiao-Qian Wu; Na Dong; Rui Ling; Hong Jiang

doi:10.1080/23802359.2019.1637288

Next-generation sequencing yields the complete chloroplast genome of Pleione forrestii (Orchidaceae)

Mitochondrial DNA B Resour. 2019 Jul 31;4(2):2777-2778. doi: 10.1080/23802359.2019.1637288.

Authors

Sha-Sha Wu^{1

2}, Xiao-Qian Wu^{1

2}, Na Dong^{1

2}, Rui Ling^{1

2}, Hong Jiang³

Affiliations

¹ Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, China.
² Fujian Ornamental Plant Germplasm Resources Innovation & Engineering Application Research Center, Fuzhou, China.
³ Yunnan Academy of Forestry, Kunming, China.

Abstract

Pleione forrestii is an endangered terrestrial orchid with significant ornamental values. Here, we report the first complete chloroplast genome of P. forrestii. The circular genome was 158,759 bp in length and consisted of a pair of inverted repeats (IR 26,429 bp), which were separated by a large single-copy region (LSC 87,236 bp) and a small single copy-region (SSC 18,665 bp). It contained 135 genes (115 unique), including 87 protein-coding genes, 38 tRNAs, and 8 rRNAs. The maximum likelihood phylogenetic analysis indicated that P. forrestii was sister to P. bulbocodioides and P. formosana.

Keywords: Chloroplast genome; Illumina sequencing; Pleione forrestii; phylogenetic.