Next-generation sequencing yields the complete chloroplast genome of Pleione chunii, a vulnerable orchid in China

Sha-Sha Wu; Li-Ming Shen; Rui Ling; Zhong-Wu Dai; Zhong-Jian Liu; Si-Ren Lan

doi:10.1080/23802359.2019.1640642

Next-generation sequencing yields the complete chloroplast genome of Pleione chunii, a vulnerable orchid in China

Mitochondrial DNA B Resour. 2019 Jul 16;4(2):2576-2578. doi: 10.1080/23802359.2019.1640642.

Authors

Sha-Sha Wu^{1

2}, Li-Ming Shen^{1

2}, Rui Ling^{1

2}, Zhong-Wu Dai^{1

2}, Zhong-Jian Liu^{1

2}, Si-Ren Lan^{1

2}

Affiliations

¹ Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, China.
² Fujian Ornamental Plant Germplasm Resources Innovation and Engineering Application Research Center, Fujian Agriculture and Forestry University, Fuzhou, China.

Abstract

Pleione chunii is a vulnerable epiphytic orchid with significant ornamental value. Here, we report the first complete chloroplast genome of P. chunii. The circular genome was 158,880 bp in length and consisted of a pair of inverted repeats (IR 26,465 bp), which were separated by a large single copy region (LSC 87,259 bp) and a small single copy region (SSC 18,691 bp). It contained 115 unique genes, including 87 protein-coding genes, 38 tRNAs, and eight rRNAs. The maximum-likelihood phylogenetic analysis indicated that P. chunii was sister to P. bulbocodioides and P. formosana.

Keywords: Chloroplast genome; Illumina sequencing; Orchidaceae; Phylogenetic; Pleione chunii.