New mitochondrial genomes of 39 soil dwelling Coleoptera from metagenome sequencing

Carmelo Andújar; Paula Arribas; Michal Motyka; Mathew Bocek; Ladislav Bocak; Benjamin Linard; Alfried P Vogler

doi:10.1080/23802359.2019.1637289

New mitochondrial genomes of 39 soil dwelling Coleoptera from metagenome sequencing

Mitochondrial DNA B Resour. 2019 Jul 12;4(2):2447-2450. doi: 10.1080/23802359.2019.1637289.

Authors

Carmelo Andújar^{1

2

3}, Paula Arribas^{1

2

3}, Michal Motyka⁴, Mathew Bocek⁴, Ladislav Bocak⁴, Benjamin Linard^{2

5}, Alfried P Vogler^{2

3}

Affiliations

¹ Grupo de Ecología y Evolución en Islas, Instituto de Productos Naturales y Agrobiología (IPNA-CSIC), San Cristóbal de la Laguna, Spain.
² Department of Life Sciences, Natural History Museum, London, UK.
³ Department of Life Sciences, Imperial College London, Ascot, UK.
⁴ Department of Zoology, Faculty of Science, Palacky University, Olomouc, Czech Republic.
⁵ LIRMM (Laboratoire d'Informatique de Robotique et de Microelectronique de Montpellier), CNRS University of Montpellier, Montpellier, France.

Abstract

High-throughput DNA methods hold great promise for the study of the hyperdiverse arthropod fauna of the soil. We used the mitochondrial metagenomic approach to generate 39 mitochondrial genomes from adult and larval specimens of Coleoptera collected from soil samples. The mitogenomes correspond to species from the families Carabidae (6), Chrysomelidae (1), Curculionidae (9), Dermestidae (1), Elateridae (1), Latridiidae (1), Scarabaeidae (3), Silvanidae (1), Staphylinidae (12), and Tenebrionidae (4). All the mitogenomes followed the putative ancestral gene order for Coleoptera. We provide the first available mitogenome for 30 genera of Coleoptera, including endogean representatives of the genera Torneuma, Coiffaitiella, Otiorhynchus, Oligotyphlopsis, and Typhlocharis.

Keywords: Coleoptera; endogean; mitochondrial metagenomics; next-generation sequencing; soil.