BMSC-Derived Exosomal miR-29a Promotes Angiogenesis and Osteogenesis

Guo-Dong Lu; Peng Cheng; Ting Liu; Zhong Wang

doi:10.3389/fcell.2020.608521

BMSC-Derived Exosomal miR-29a Promotes Angiogenesis and Osteogenesis

Front Cell Dev Biol. 2020 Dec 9:8:608521. doi: 10.3389/fcell.2020.608521. eCollection 2020.

Authors

Guo-Dong Lu¹, Peng Cheng², Ting Liu³, Zhong Wang¹

Affiliations

¹ Department of Cardiology, The First Affiliated Hospital of Shihezi University Medical College, Shihezi, China.
² Division of Geriatric Endocrinology, The First Affiliated Hospital, Nanjing Medical University, Nanjing, China.
³ Department of Endocrinology, Changsha Central Hospital, Changsha, China.

Abstract

Angiogenesis and osteogenesis are tightly coupled during bone modeling and remodeling processes. Here we reported that bone marrow mesenchymal stem cell (BMSC)-derived exosomal miR-29a promotes angiogenesis and osteogenesis in vitro and in vivo. BMSC-derived exosomes (BMSCs-Exos) can be taken up by human umbilical vein endothelial cells (HUVECs) and promote the proliferation, migration, and tube formation of HUVECs. MiRNA-29a level was high in BMSCs-Exos and can be transported into HUVECs to regulate angiogenesis. VASH1 was identified as a direct target of miR-29a, mediating the effects of BMSC-derived exosomal miR-29a on angiogenesis. More interestingly, miR29a-loaded exosomes from engineered BMSCs (miR-29a-loaded BMSCs-Exos) showed a robust ability of promoting angiogenesis and osteogenesis in vivo. Taken together, these findings suggest that BMSC-derived exosomal miR-29a regulates angiogenesis and osteogenesis, and miR-29a-loaded BMSCs-Exos may serve as a potential therapeutic target for osteoporosis.

Keywords: BMSCs-exosomes; angiogenesis; miRNA-29a; osteogenesis; osteoporosis.