Shunt products of aminoansamycins from aas1 overexpressed mutant strain of Streptomyces sp. S35

Li-Ping Dai; Zi-Shen Wang; Hao-Xin Wang; Chun-Hua Lu; Yue-Mao Shen

doi:10.1016/S1875-5364(20)60039-5

Shunt products of aminoansamycins from aas1 overexpressed mutant strain of Streptomyces sp. S35

Chin J Nat Med. 2020 Dec;18(12):952-956. doi: 10.1016/S1875-5364(20)60039-5.

Authors

Li-Ping Dai¹, Zi-Shen Wang², Hao-Xin Wang², Chun-Hua Lu³, Yue-Mao Shen⁴

Affiliations

¹ Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China.
² State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China.
³ Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China. Electronic address: ahua0966@sdu.edu.cn.
⁴ Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China; State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China. Electronic address: yshen@sdu.edu.cn.

PMID: 33357726
DOI: 10.1016/S1875-5364(20)60039-5

Abstract

Constitutively expression of the pathway-specific activators is an effective method to activate silent gene clusters and improve natural product production. In this study, nine shunt products of aminoansamycins (1-9) were identified from a recombinant mutant strain S35-LAL by overexpressed the large-ATP-binding regulator of the LuxR family (LAL) gene aas1 in Streptomyces sp. S35. All the compounds showed no anti-microbial, anti-T3SS and cytotoxic activities.

Keywords: Aminoansamycins; Shunt products; Streptomyces sp. S35.

MeSH terms

Biological Products / metabolism*
Lactams, Macrocyclic / metabolism*
Multigene Family*
Organisms, Genetically Modified
Streptomyces / genetics*
Streptomyces / metabolism

Substances

Biological Products
Lactams, Macrocyclic