Solid-State Fermentation of Aspergillus niger to Optimize Extraction Process of Isoliquiritigenin from Glycyrrhiza uralensis

Jingwei Hao; Dianwei Li; Yunrong Jing; Lei Zhang; Jiahui Liu; Yubin Ji

doi:10.1155/2020/8927858

Solid-State Fermentation of Aspergillus niger to Optimize Extraction Process of Isoliquiritigenin from Glycyrrhiza uralensis

Evid Based Complement Alternat Med. 2020 Nov 28:2020:8927858. doi: 10.1155/2020/8927858. eCollection 2020.

Authors

Jingwei Hao^{1

2}, Dianwei Li², Yunrong Jing², Lei Zhang², Jiahui Liu², Yubin Ji¹

Affiliations

¹ Center for Life Science and Enviromental Science, Harbin University of Commerce, Harbin 150076, China.
² College of Life Sciences and Technology, Mudanjiang Normal University, Mudanjiang 157011, China.

Abstract

We successfully extracted isoliquiritigenin from Glycyrrhiza uralensis via fermentation with Aspergillus niger and ultrasonic-assisted extraction. In brief, we used A. niger fermentation to culture G. uralensis powder, and we optimized some key parameters such as reaction conditions of pH, inoculation concentration of A. niger, fermentation time, and solid-liquid ratio. Based on a single-factor experiment, we utilized the response surface methodology (RSM) approach to optimize this extraction procedure. Using the RSM approach, optimized conditions of pH = 3.694, the solid-liquid ratio = 1 : 2.155, and the inoculation concentration of A. niger = 1466745 were selected. Optimized conditions resulted in an extraction efficiency of 1.525 mg/g. These results showed that the extraction of isoliquiritigenin was most affected by pH and then the time of fermentation and the solid-liquid ratio. Overall, the developed extraction technique yielded 5 times the amount of isoliquiritigenin when compared to traditional methods.