Ratiometric nanoprobe for circulating tumor cell detection and intracellular hydrogen peroxide evaluation in colorectal cancer patients

Abstract

The application of intensity-based H₂O₂-responsive fluorescence nanoprobe for circulating tumor cell detection was limited by the complex background and the nanoprobe uptake in each CTC. In this context, we developed a ratiometric fluorescence nanoprobe, on which a H₂O₂-responsive subunit and a stable subunit grafted working as a H₂O₂ detector and a reference, respectively. When responding to intracellular H₂O₂, the reference fluorescence (580 nm) maintained as a correction background while the detector fluorescence (450 nm) was turned on to conduct CTC enumeration and intracellular H₂O₂ evaluation. Two normal cells and three colon cancer cells were examined to evaluate their endogenous H₂O₂ with the ratiometric nanoprobe by flow cytometry and confocal laser scanning microscopy. CTC sample from colorectal cancer patients was used to validate the performance of the nanoprobe for CTC enumeration and H₂O₂ evaluation. The results indicated that not only CTC could be effectively identified based on the "turn on" fluorescence, but also the viability of the identified CTCs could be assessed with the intensity of the reference fluorescence to avoid the false-positive number. Moreover, the clinical results demonstrated that the viability CTC count combined with intracellular H₂O₂ content (described as I₄₅₀/₅₈₀)were related to the tumor TNM stage, which might provide significant guidance for clinical treatments.

Keywords: Circulating tumor cancer; Hydrogen peroxide; Ratiometric nanoprobe.