Bio-clogging is a major problem in the operation of constructed wetlands (CWs) and is caused by accumulation of biofilm and extracellular polymeric substances (EPS) in the substrate. B. subtilis can successfully produce α-amylase and endoglucanase, which can degrade polysaccharides and, consequently, disperse the EPS. Therefore, the addition of B. subtilis was used to decrease the bio-clogging of lab-scale vertical-flow constructed wetlands (VFCW) in this study, and the feasibility and performance of VFCWs were assessed. The results indicate that the addition of B. subtilis can degrade the polysaccharides in the clogging matter and thereby increase the porosity of the substrate. The hydraulic conductivity of Column 1 (with addition) increased by six times, which was 57 times that of control (Column 2). Meanwhile, the chemical oxygen demand (COD) removal rate also increased after the addition of B. subtilis. The microbial communities show that the richness and diversity within the substrate increased after addition. The relative abundance of functional groups of chemoheterotrophy, aerobic chemoheterotrophy, as well as that connected to N cycles also increased, which implied the improvement of the pollution removal efficiency. Meanwhile, the copy number of α-amylase and endoglucanase increased significantly in Column 1 with the addition of B. subtilis, which offers further support for a hydrolase-induced reduction of polysaccharides and the efficiency of B. subtilis on bio-clogging alleviation. The results showed that B. subtilis addition is an effective and safe solution to control the bio-clogging for CWs. However, further research about long-term effect assessment and dosing strategy optimization should be conducted.
Keywords: B. subtilis; Bio-clogging; Constructed wetland; Microbial community.
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