A newly synthesized visual bis(salamo)-based fluorescent chemosensor for exogenous detection of B4O72- in living cells and zebrafish

Abstract

A newly synthesized fluorescent chemosensor H₆L was explored for detecting B₄O₇^2-, characterized by ¹H NMR spectrum, mass spectrum and fluorescence spectra. During the detection process of B₄O₇^2-, the fluorescence is significantly enhanced and naked eye recognition can be performed under 365 nm UV light without any interference by other typical anions. The limit of detection is as low as 6.97 × 10^-10 M. In addition, in order to broaden the application of salamo-based fluorescence sensors in the field of biology, except for the fluorescence imaging of HeLa cells, the first attempt of exogenous detection in zebrafish was conducted successfully.

Keywords: Deprotonation effect; Exogenous detection; Fluorescent chemosensor; Zebrafish cultivate.