Super-Enhancer LncRNA LINC00162 Promotes Progression of Bladder Cancer

Xin Wang; Ruirui Zhang; Shuilian Wu; Liping Shen; Meixia Ke; Yan Ouyang; Mengqi Lin; Yiting Lyu; Binuo Sun; Zhijian Zheng; Jialei Yang; Jie Yang; Wenmin Lu; Yiping Yang; Danni Li; Yunfeng Zou; Haishan Huang; Aruo Nan

doi:10.1016/j.isci.2020.101857

Super-Enhancer LncRNA LINC00162 Promotes Progression of Bladder Cancer

iScience. 2020 Nov 24;23(12):101857. doi: 10.1016/j.isci.2020.101857. eCollection 2020 Dec 18.

Authors

Xin Wang¹, Ruirui Zhang^{1

2

3}, Shuilian Wu¹, Liping Shen¹, Meixia Ke¹, Yan Ouyang¹, Mengqi Lin¹, Yiting Lyu¹, Binuo Sun¹, Zhijian Zheng¹, Jialei Yang³, Jie Yang^{2

3}, Wenmin Lu^{2

3}, Yiping Yang^{2

3}, Danni Li^{2

3}, Yunfeng Zou^{2

3}, Haishan Huang¹, Aruo Nan^{1

2

3}

Affiliations

¹ Zhejiang Provincial Key Laboratory of Medical Genetics, Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325035, China.
² Department of Toxicology, School of Public Health, Guangxi Medical University, Nanning 530021, China.
³ Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases, Guangxi Medical University, Nanning 530021, China.

Abstract

Due to the lack of effective early diagnostic measures and treatment methods, bladder cancer has become a malignant tumor that seriously threatens people's lives and health. Here, we reported that LINC00162, a super-enhancer long noncoding RNA, was highly expressed in bladder cancer cells and tissues. And LINC00162 was negatively correlated with neighboring PTTG1IP expression. Knocking down LINC00162 expression can inhibit the proliferative activity of bladder cancer cells and the growth of transplanted tumors in vivo, while knocking down the expression of PTTG1IP could restore the proliferative activity of bladder cancer cells. In addition, both LINC00162 and PTTG1IP were found to be able to bind to THRAP3, a transcription-related protein. And THRAP3 can regulate PTTG1IP expression. Finally, we demonstrated a mechanism that LINC00162 could regulate PTTG1IP expression through binding THRAP3. This study provided a potential target molecule for clinical treatment of bladder cancer.

Keywords: Cancer; Molecular Biology.