Color Tuning of Fluorogens for FAST Fluorogen-Activating Protein

Abstract

Using benzylidene imidazolone core, we created a panel of color-shifted fluorogenic ligands for FAST protein without compromise to the binding efficiency and the utility for live-cell protein labeling. This study highlights the potential of benzylidene imidazolones derivatives for rapid expansion of a pallet of live-cell fluorogenic labeling tools.

Keywords: chromophore; fluorescence; fluorescent labeling; fluorogen-activating proteins; green fluorescent protein.