Abstract
Cyclopentane modified FIT-PNA (cpFIT-PNA) probes are reported as highly emissive RNA sensors with the highest reported brightness for FIT-PNAs. Compared to FIT-PNAs, cpFIT-PNAs have improved mismatch discrimination for several pyrimidine-pyrimidine single nucleotide variants (SNVs).
MeSH terms
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Benzothiazoles / chemistry*
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Cyclopentanes / chemistry*
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Fluorescent Dyes / chemistry*
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Limit of Detection
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Nucleic Acid Hybridization
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Peptide Nucleic Acids / chemistry*
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Peptide Nucleic Acids / genetics
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Quinolines / chemistry*
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RNA / analysis*
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RNA / genetics
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Spectrometry, Fluorescence
Substances
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Benzothiazoles
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Cyclopentanes
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Fluorescent Dyes
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Peptide Nucleic Acids
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Quinolines
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thiazole orange
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RNA