A novel Ag/carrageenan-gelatin hybrid hydrogel nanocomposite and its biological applications: Preparation and characterization

Lakshmanan Muthulakshmi; U Pavithra; V Sivaranjani; N Balasubramanian; Kunnathur Murugesan Sakthivel; Catalin Iulian Pruncu

doi:10.1016/j.jmbbm.2020.104257

A novel Ag/carrageenan-gelatin hybrid hydrogel nanocomposite and its biological applications: Preparation and characterization

J Mech Behav Biomed Mater. 2021 Mar:115:104257. doi: 10.1016/j.jmbbm.2020.104257. Epub 2020 Dec 9.

Authors

Lakshmanan Muthulakshmi¹, U Pavithra², V Sivaranjani², N Balasubramanian³, Kunnathur Murugesan Sakthivel⁴, Catalin Iulian Pruncu⁵

Affiliations

¹ Department of Materials Science, School of Chemistry, Madurai Kamaraj University, Madurai, 625021, Tamil Nadu, India; Department of Biotechnology, Kalasalingam Academy of Research and Education, Anand Nagar, Krishnankoil, 626126, Tamil Nadu, India. Electronic address: mthlakshmi27@gmail.com.
² Department of Biotechnology, Kalasalingam Academy of Research and Education, Anand Nagar, Krishnankoil, 626126, Tamil Nadu, India.
³ Department of Immunology, School of Biological Sciences, Madurai Kamaraj University, Madurai, 625021, Tamil Nadu, India.
⁴ Department of Biotechnology, Regional Cancer Centre, Thiruvananthapuram, 695011, Kerala, India; Department of Biochemistry, PSG College of Arts and Science, Coimbatore 641014, Tamilnadu, India.
⁵ Department of Mechanical Engineering, Imperial College London, Exhibition Rd., London, SW7 2AZ, UK; Design, Manufacturing & Engineering Management, University of Strathclyde, Glasgow, G1 1XJ, Scotland, UK. Electronic address: Catalin.pruncu@strath.ac.uk.

PMID: 33333481
DOI: 10.1016/j.jmbbm.2020.104257

Abstract

A novel biohybrid hydrogel nanocomposite made of natural polymer carrageenan and gelatin protein were developed. The silver nanoparticles were prepared using the carrageenan polymer as reduction and capping agent. Here, the Ag/Carrageenan was combined with gelatin hydrogel using glutaraldehyde having a cross-link role in order to create the biohybrid hydrogel nanocomposite. The manufactured composite performances were anaylised by UV-visible spectroscopy, Fourier Transform infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), Energy dispersive X-ray (EDX) spectroscopy and Transmission Electron Microscopy (TEM) methods. The swelling behaviour of the Ag/Carrageenan-gelatin hybrid hydrogel nanocomposite was also analyzed. The antibacterial activity was tested against human pathogens viz. S.agalactiae 1661, S. pyogenes 1210 and E. coli. The bacterial cell wall damage of S.agalactiae 1661 was analyzed by scanning electron microscopy. The cytotoxic assay was performed against the A549 lung cancer cells.

Keywords: Biocompatibility; Capping agent; Carrageenan; Hydrogel; Polysaccharide.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
Carrageenan
Escherichia coli
Gelatin
Humans
Hydrogels
Metal Nanoparticles*
Microscopy, Electron, Scanning
Nanocomposites*
Silver
Spectroscopy, Fourier Transform Infrared
X-Ray Diffraction

Substances

Anti-Bacterial Agents
Hydrogels
Silver
Carrageenan
Gelatin