Bioassay-guided isolation of anti-inflammatory constituents from Celtis sinensis leaves

Zhipeng Qi; Xianying Fang; Yingying Xie; Lei Wang; Yiwei Zhang; Linguo Zhao

doi:10.1111/jfbc.13580

Bioassay-guided isolation of anti-inflammatory constituents from Celtis sinensis leaves

J Food Biochem. 2021 Jan;45(1):e13580. doi: 10.1111/jfbc.13580. Epub 2020 Dec 16.

Authors

Zhipeng Qi^{1

2}, Xianying Fang^{1

2}, Yingying Xie^{1

2}, Lei Wang^{1

2}, Yiwei Zhang^{1

2}, Linguo Zhao^{1

2}

Affiliations

¹ Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.
² College of Chemical Engineering, Nanjing Forestry University, Nanjing, China.

PMID: 33326628
DOI: 10.1111/jfbc.13580

Abstract

Ginkgo acids (GAs) in ginkgo products usually lead to allergies or liver toxicity. In this study, the GA-induced toxicity was attenuated and Con A-induced T-lymphocyte proliferation was inhibited by extracts of Celtis sinensis leaves (ECSL). So, the active ingredients in ECSL were studied to solve the problems caused by GAs. First, the eight components of MeOH extracts were determined by HPLC-DAD/LC-MS. Then, the 12 active ingredients were separated based on the anti-inflammatory activity. Lymphocyte conversion showed that the inhibition rates of apigenin, quercetin, and isovitexin at 100 μM on Con A-activated proliferation of T cells were up to 82.46%, 62.86% and 42.76%, respectively. The inhibition rate on the LPS-induced NO release in RAW 264.7 cells of quercetin, apigenin, isovitexin, and vitexin were exceeding 80% at 100 μM. Taken together, the material foundation for the screen of GAs toxicity-attenuated ingredients were provided here. PRACTICAL APPLICATIONS: Ginkgo biloba extracts (EGBs) have been conducted to develop functional food which could increase blood circulation and enhance memory. Nevertheless, people in consumption of ginkgo products, often caused severe allergic reactions due to the potential allergens identified ginkgolic acids (GAs) of ginkgo products. We first find that the extracts of Celtis sinensis leaves can reduce GAs-induced damage on HepG2 liver cells. Then, the bioactive compounds in C. sinensis leaves were separated and purified based on anti-inflammatory activities against T cells. Quercetin, apigenin, and isovitexin showed well anti-inflammatory activities against Con A-activated T-lymphocytes and LPS activated RAW 264.7 macrophages. However, quercetin and apigenin are flavones O-glycosides which are rich in Ginkgo biloba. To solve the problems in Ginkgo biloba products caused by GAs, flavone C-glycoside (isovitexin) may be used for the further study in GAs toxicity-reduction.

Keywords: Celtis sinensis leaves; anti-inflammatory; cytotoxicity; ginkgolic acids; structure characterization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Inflammatory Agents / pharmacology
Biological Assay
Ginkgo biloba*
Plant Leaves*
Ulmaceae

Substances

Anti-Inflammatory Agents