Highly Selective Optical Sensor Eu (TTA)3 Phen Embedded in Poly Methylmethacrylate for Assessment of Total Prostate Specific Antigen Tumor Marker in Male Serum Suffering Prostate Diseases

Mohannad Garoub; A H Hefny; W E Omer; Mostafa M Elsaady; Mohamed M Abo-Aly; Ali A Sayqal; Ahmed Alharbi; Ahmed Hameed; Hussain Alessa; A O Youssef; Ekram H Mohamed; Ayman A Gouda; R El Sheikh; M N Abou-Omar; Maged A El-Kemary; M S Attia

doi:10.3389/fchem.2020.561052

Highly Selective Optical Sensor Eu (TTA)₃ Phen Embedded in Poly Methylmethacrylate for Assessment of Total Prostate Specific Antigen Tumor Marker in Male Serum Suffering Prostate Diseases

Front Chem. 2020 Nov 19:8:561052. doi: 10.3389/fchem.2020.561052. eCollection 2020.

Authors

Mohannad Garoub¹, A H Hefny², W E Omer³, Mostafa M Elsaady¹, Mohamed M Abo-Aly¹, Ali A Sayqal⁴, Ahmed Alharbi⁴, Ahmed Hameed⁴, Hussain Alessa⁴, A O Youssef¹, Ekram H Mohamed⁵, Ayman A Gouda^{2

6}, R El Sheikh⁶, M N Abou-Omar⁷, Maged A El-Kemary³, M S Attia¹

Affiliations

¹ Chemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
² Occupational Health Department, Faculty of Public Health and Health Informatics, Umm AL Qura University, Makkah, Saudi Arabia.
³ Institute of Nanoscience & Nanotechnology, Kafrelsheikh University, Kafr ElSheikh, Egypt.
⁴ Department of Chemistry, Faculty of Applied Science, Umm Al-Qura University, Makkah, Saudi Arabia.
⁵ Department of Analytical Chemistry, Faculty of Pharmacy, The British University in Egypt (BUE), Cairo, Egypt.
⁶ Chemistry Department, Faculty of Science, Zagazig University, Zagazig, Egypt.
⁷ Department of Chemistry, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo, Egypt.

Abstract

A low-cost, simple, and highly selective method was used for the assessment of total prostate specific antigen (tPSA) in the serum of prostate cancer patients. This method is based on quenching the intensity of luminescence displayed by the optical sensor Eu (TTA)₃ phen/poly methylmethacrylate (PMMA) thin membrane or film upon adding different concentrations of tPSA. The luminescent optical sensor was synthesized and characterized through absorption, emission, scanning electron microscopy (SEM), and x-ray diffraction (XRD), and is tailored to present red luminescence at 614 nm upon excitation at 395 nm in water. The fabricated sensor fluorescence intensity is quenched in the presence of tPSA in aqueous media. The fluorescence resonance energy transfer (FRET) is the main mechanism by which the sensor performs. The sensor was successfully utilized to estimate tPSA in the serum of patients suffering prostate cancer in a time and cost effective way. The statistical results of the method were satisfactory with 0.0469 ng mL^-1 as a detection limit and 0.99 as a correlation coefficient.

Keywords: luminescence; optical sensor; prostate cancer; quenching; specific antigen.