Abstract
Circular RNAs (circRNAs) are associated with chemoresistance in many cancers. However, the function of circ_0005198 in the temozolomide (TMZ) resistance of glioma has not been well elucidated. Here, we demonstrated that circ_0005198 was considerably up-regulated in glioma tissues, serum samples and TMZ-resistant glioma cells. Silencing of circ_0005198 restrained TMZ resistance, restricted the proliferation and facilitated the apoptosis of TMZ-resistant glioma cells. MiR-198 could be sponged by circ_0005198, and we demonstrated that the effect of circ_0005198 on the progression of TMZ-resistant glioma cells was attributed to the inhibition of miR-198 activity. Moreover, TRIM14 was a target of miR-198 and silencing of TRIM14 hindered TMZ resistance and suppressed the progression of TMZ-resistant glioma cells, while TRIM14 over-expression rescued the inhibiting effect of miR-198 over-expression. We conclude that circ_0005198-miR-198-TRIM14 regulatory pathway is critical to TMZ resistance of glioma.
Keywords:
TMZ resistance; TRIM14; circ_0005198; glioma; miR-198.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents, Alkylating / pharmacology*
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Antineoplastic Agents, Alkylating / therapeutic use
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Apoptosis / drug effects
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Brain Neoplasms / drug therapy
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Brain Neoplasms / genetics*
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Brain Neoplasms / metabolism
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Brain Neoplasms / pathology
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Cell Line, Tumor
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Cell Proliferation / drug effects
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Drug Resistance, Neoplasm / drug effects
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Drug Resistance, Neoplasm / genetics*
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Gene Expression Regulation, Neoplastic / drug effects
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Glioma / drug therapy
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Glioma / genetics*
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Glioma / metabolism
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Glioma / pathology
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Humans
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Intracellular Signaling Peptides and Proteins / genetics
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Intracellular Signaling Peptides and Proteins / metabolism*
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MicroRNAs / genetics
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MicroRNAs / metabolism*
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RNA, Circular / genetics
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RNA, Circular / metabolism*
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Temozolomide / pharmacology*
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Temozolomide / therapeutic use
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Tripartite Motif Proteins / genetics
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Tripartite Motif Proteins / metabolism*
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Up-Regulation / drug effects
Substances
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Antineoplastic Agents, Alkylating
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Intracellular Signaling Peptides and Proteins
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MIRN198 microRNA, human
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MicroRNAs
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RNA, Circular
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TRIM14 protein, human
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Tripartite Motif Proteins
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Temozolomide