Label-free DNAzyme assays for dually amplified and one-pot detection of lead pollution

Yong Zhang; Chengyong Wu; Hongxin Liu; Mohammad Rizwan Khan; Zhifeng Zhao; Guiping He; Aimin Luo; Jiaqi Zhang; Ruijie Deng; Qiang He

doi:10.1016/j.jhazmat.2020.124790

Label-free DNAzyme assays for dually amplified and one-pot detection of lead pollution

J Hazard Mater. 2021 Mar 15:406:124790. doi: 10.1016/j.jhazmat.2020.124790. Epub 2020 Dec 7.

Authors

Affiliations

¹ College of Biomass Science and Engineering, Healthy Food Evaluation Research Center and Key Laboratory of Food Science and Technology of Ministry of Education of Sichuan Province, Sichuan University, Chengdu 610065, China.
² State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan 610041, China.
³ Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
⁴ College of Biomass Science and Engineering, Healthy Food Evaluation Research Center and Key Laboratory of Food Science and Technology of Ministry of Education of Sichuan Province, Sichuan University, Chengdu 610065, China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 10048, China.
⁵ College of Biomass Science and Engineering, Healthy Food Evaluation Research Center and Key Laboratory of Food Science and Technology of Ministry of Education of Sichuan Province, Sichuan University, Chengdu 610065, China. Electronic address: drj17@scu.edu.cn.

PMID: 33316668
DOI: 10.1016/j.jhazmat.2020.124790

Abstract

Lead pollution in water and soil often transfers to food, advocating tools for on-site detection of lead pollution to ensure both environmental and food safety. We proposed a label-free, dually amplified and homogeneous DNAzyme assay for sensitive and one-pot detection of lead pollution. Instead of using chemically modified DNA substrate, a structure-response digestion process was introduced to monitor Pb²⁺ presence-induced cleavage process of unlabeled substrate, further amplifying the response signals and eliminating the use of labeled DNA probes. The DNAzyme assay allowed to detect Pb²⁺ as low as 0.12 nM and endued a dynamic range from 0.1 nM to 30 nM. In addition, it can specifically identify Pb²⁺ among other metal ions. We demonstrated that the DNAzyme assay can precisely detect Pb²⁺ in tap water, milk and fish. Thus, the DNAzyme assay is promising for on-site monitoring lead pollution risk and ensuring environmental and food safety.

Keywords: Amplification; DNAzyme; Fluorescence assay; Label-free; Lead pollution.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biosensing Techniques*
DNA, Catalytic*
Drinking Water
Environmental Pollutants / analysis*
Fishes
Food Contamination
Ions
Lead / analysis*
Limit of Detection
Milk

Substances

DNA, Catalytic
Drinking Water
Environmental Pollutants
Ions
Lead