Performance evaluation of a selective-enrichment-isolation protocol for Salmonella enterica from seafood

Parmanand Prabhakar; Manjusha Lekshmi; Toms C Joseph; Amjad K Balange; Binaya Bhusan Nayak; Sanath H Kumar

doi:10.1016/j.mimet.2020.106120

Performance evaluation of a selective-enrichment-isolation protocol for Salmonella enterica from seafood

J Microbiol Methods. 2021 Jan:180:106120. doi: 10.1016/j.mimet.2020.106120. Epub 2020 Dec 13.

Authors

Parmanand Prabhakar¹, Manjusha Lekshmi¹, Toms C Joseph², Amjad K Balange¹, Binaya Bhusan Nayak¹, Sanath H Kumar³

Affiliations

¹ QC Laboratory, Post Harvest Technology, ICAR-Central Institute of Fisheries Education, Versova, Mumbai 400061, India.
² ICAR-Central Institute of Fisheries Technology, Veraval Research Centre, Veraval 362269, India.
³ QC Laboratory, Post Harvest Technology, ICAR-Central Institute of Fisheries Education, Versova, Mumbai 400061, India. Electronic address: sanathkumar@cife.edu.in.

PMID: 33316291
DOI: 10.1016/j.mimet.2020.106120

Abstract

In this study using 57 finfish samples of marine origin, selective enrichment in Rappaport-Vassiliadis (RV) broth followed by isolation on the Hektoen enteric agar (HEA) yielded 50 (53.2%) of 94 isolates. The results suggest RV-HEA as the most suitable media combination for the recovery of Salmonella from tropical seafood.

Keywords: Enrichment; Fish; Isolation; Salmonella; Seafood; cCulture media.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Agar
Animals
Bacteriological Techniques / methods*
Culture Media / chemistry
Fishes / microbiology
Food Microbiology / methods*
Salmonella / isolation & purification
Salmonella enterica / isolation & purification*
Seafood / microbiology*

Substances

Culture Media
Agar