Thioridazine hydrochloride: an antipsychotic agent that inhibits tumor growth and lung metastasis in triple-negative breast cancer via inducing G0/G1 arrest and apoptosis

Yanlin Song; Lu Li; Jiao Chen; Hongli Chen; Bomiao Cui; Yun Feng; Ping Zhang; Qiangsheng Zhang; Yong Xia; Min Luo

doi:10.1080/15384101.2020.1850969

Thioridazine hydrochloride: an antipsychotic agent that inhibits tumor growth and lung metastasis in triple-negative breast cancer via inducing G0/G1 arrest and apoptosis

Cell Cycle. 2020 Dec;19(24):3521-3533. doi: 10.1080/15384101.2020.1850969. Epub 2020 Dec 14.

Authors

Yanlin Song^{1

2}, Lu Li¹, Jiao Chen¹, Hongli Chen¹, Bomiao Cui¹, Yun Feng¹, Ping Zhang¹, Qiangsheng Zhang¹, Yong Xia^{3

4}, Min Luo¹

Affiliations

¹ State Key Laboratory of Oral Disease, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University , Chengdu, Sichuan, China.
² West China School of Medicine, West China Hospital, Sichuan University , Chengdu, Sichuan, China.
³ Department of Rehabilitation Medicine, West China Hospital, Sichuan University , Chengdu, Sichuan, 610041, China.
⁴ Key Laboratory of Rehabilitation Medicine, West China Hospital, Sichuan University , Chengdu Sichuan, 610041, China.

Abstract

CCK8: Cell Counting Kit-8; CDK: cyclin-dependent kinase; DRD2: dopamine D2 receptor; ERK1/2: extracellular signal-regulated kinase 1/2; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; H&E: hematoxylin and eosin; MMP: membrane potential; NAC: N-acetyl-L-cysteine; PI: Propidium iodide; Rh123: rhodamine-123; ROS: reactive oxygen species; TBST: tris-buffered saline containing 0.1% Tween 20 TNBC: Triple-negative breast cancer; Thi-hyd: Thioridazine hydrochloride.

Keywords: PI3K/AKT pathway; Thioridazine hydrochloride; antipsychotic agent; triple-negative breast cancer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antipsychotic Agents / pharmacology*
Antipsychotic Agents / therapeutic use
Apoptosis / drug effects*
Cell Line, Tumor
Cell Movement / drug effects
Cell Proliferation / drug effects*
Cell Survival / drug effects
Female
G1 Phase Cell Cycle Checkpoints / drug effects*
Humans
Lung Neoplasms / metabolism*
Lung Neoplasms / secondary*
Membrane Potential, Mitochondrial / drug effects
Mice
Mice, Inbred BALB C
Reactive Oxygen Species / metabolism
Resting Phase, Cell Cycle / drug effects*
Signal Transduction / drug effects
Thioridazine / pharmacology*
Thioridazine / therapeutic use
Treatment Outcome
Triple Negative Breast Neoplasms / drug therapy
Triple Negative Breast Neoplasms / metabolism*
Triple Negative Breast Neoplasms / pathology
Xenograft Model Antitumor Assays

Substances

Antipsychotic Agents
Reactive Oxygen Species
Thioridazine

Grants and funding

This study was funded by grants from the National Natural Science Foundation of China [31800773], China Postdoctoral Science Foundation [2018M633369], Sichuan Science and Technology Program [2019YJ0063], and The Youth Science Foundation of West China Hospital of Stomatology [WCHS-201703] to M.L.