A novel near-infrared fluorimetric method for point-of-care monitoring of Fe2+ and its application in bioimaging

Meiqing Zhu; Zongyuan Zhao; Xina Liu; Panpan Chen; Fugang Fan; Xiangwei Wu; Rimao Hua; Yi Wang

doi:10.1016/j.jhazmat.2020.124767

A novel near-infrared fluorimetric method for point-of-care monitoring of Fe²⁺ and its application in bioimaging

J Hazard Mater. 2021 Mar 15:406:124767. doi: 10.1016/j.jhazmat.2020.124767. Epub 2020 Dec 5.

Authors

Meiqing Zhu¹, Zongyuan Zhao¹, Xina Liu¹, Panpan Chen¹, Fugang Fan¹, Xiangwei Wu¹, Rimao Hua², Yi Wang³

Affiliations

¹ Key Laboratory of Agri-Food Safety of Anhui Province, School of Resources and Environment, Anhui Agricultural University, No. 130 Changjiang West Road, Hefei 230036, China.
² Key Laboratory of Agri-Food Safety of Anhui Province, School of Resources and Environment, Anhui Agricultural University, No. 130 Changjiang West Road, Hefei 230036, China. Electronic address: rimaohua@ahau.edu.cn.
³ Key Laboratory of Agri-Food Safety of Anhui Province, School of Resources and Environment, Anhui Agricultural University, No. 130 Changjiang West Road, Hefei 230036, China; Department of Entomology and Nematology and UCD Comprehensive Cancer Center, University of California, Davis, CA 95616, USA. Electronic address: wangyi1987@cau.edu.cn.

PMID: 33310335
DOI: 10.1016/j.jhazmat.2020.124767

Abstract

Iron is one of the essential trace elements in the human body, which is involved in many important physiological processes of life. The abnormal amount of iron in the body will bring many diseases. Therefore, a novel near-infrared fluorimetric method was developed. The method is based on a fluorescent probe (E)-4-(2-(3-(dicyanomethylene)-5,5-dimethylcyclohex-1-en-1-yl)vinyl)-N, N-diethylaniline oxide (DDED) which uses N-oxide as a recognition group to real-time monitoring and imaging of Fe²⁺ in vivo and in vitro. The method exhibits excellent selectivity and high sensitivity (LOD = 27 nM) for Fe²⁺, fast reaction rate (< 4 min), extremely large Stokes shift (＞ 275 nm), low cytotoxicity. The strip test strongly illustrates the potential application of DDED in real environment. In particular, DDED has been successfully applied to real-time monitoring and imaging of Fe²⁺ in HepG² cells and zebrafish. That is, the method has great potential for the detection of Fe²⁺ in living systems.

Keywords: DFT; Extremely large stokes shift; Fe(2+); Imaging; Near-infrared fluorescent probe.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Fluorescent Dyes
Fluorometry
HeLa Cells
Humans
Point-of-Care Systems*
Spectrometry, Fluorescence
Zebrafish*

Substances

Fluorescent Dyes