Liquid carbohydrate intake modifies transsulfuration pathway both in pregnant rats and in their male descendants

Abstract

Introduction: Fructose, alone or in combination with glucose, has been used as a source of added sugars to manufacture sugary drinks and processed foods. High consumption of simple sugars, mainly fructose, has been demonstrated to be one of the causes of developing metabolic diseases. Maternal nutrition is a key factor in the health of the progeny when adult. However, ingestion of fructose-containing foods is still permitted during gestation. Hydrogen sulphide (H₂S) is a gasotransmitter produced in the transsulfuration pathway with proved beneficial effects to combat metabolic diseases.

Methods: Carbohydrates were supplied to pregnant rats in drinking water (10% wt/vol) throughout gestation, and the pregnant rats, their foetuses, and adult male descendants were studied. Later, adult male progeny from control, fructose- and glucose-fed mothers were subjected to liquid fructose, and were compared to the control group. Liver H₂S production was determined.

Results: This study shows that, in pregnancy, either a fructose-rich diet per se or situations that produce an impaired insulin sensitivity such as an excessive intake of glucose, decrease hepatic and placental production of H₂S. Furthermore, this effect was also observed in the liver of male offspring (both in foetal and adult stages). Interestingly, when these adult descendants were subjected to a high fructose intake, decreases in H₂S synthesis in liver and adipose tissue due to this fructose intake were maternal consumption dependent.

Conclusions: Given H₂S is a protective agent against diseases such as diabetes, obesity, cardiovascular diseases, and metabolic syndrome, the fact that carbohydrate consumption reduces H₂S synthesis both in pregnancy and in their progeny could have clear and relevant clinical implications.

Keywords: Adipose tissue; Foetal programming; Fructosa; Fructose; Glucosa; Glucose; Hydrogen sulfide; Hígado; Liver; Programación fetal; Sulfuro de hidrógeno; Tejido adiposo.