Mycoviral diversity and characteristics of a negative-stranded RNA virus LeNSRV1 in the edible mushroom Lentinula edodes

Mengpei Guo; Guiyu Shen; Jinjie Wang; Mingjie Liu; Yinbing Bian; Zhangyi Xu

doi:10.1016/j.virol.2020.11.008

Mycoviral diversity and characteristics of a negative-stranded RNA virus LeNSRV1 in the edible mushroom Lentinula edodes

Virology. 2021 Mar:555:89-101. doi: 10.1016/j.virol.2020.11.008. Epub 2020 Dec 4.

Authors

Mengpei Guo¹, Guiyu Shen¹, Jinjie Wang¹, Mingjie Liu¹, Yinbing Bian², Zhangyi Xu³

Affiliations

¹ Institute of Applied Mycology, Huazhong Agricultural University, Wuhan, 430070, China.
² Institute of Applied Mycology, Huazhong Agricultural University, Wuhan, 430070, China; Key Laboratory of Agro-Microbial Resource and Development (Ministry of Agriculture), Huazhong Agricultural University, Wuhan, 430070, China.
³ Institute of Applied Mycology, Huazhong Agricultural University, Wuhan, 430070, China; Key Laboratory of Agro-Microbial Resource and Development (Ministry of Agriculture), Huazhong Agricultural University, Wuhan, 430070, China. Electronic address: zyxu2016@hotmail.com.

PMID: 33308828
DOI: 10.1016/j.virol.2020.11.008

Abstract

Bioinformatics and RT-PCR analysis of RNA from four Lentinula edodes samples identified 22 different virus-like contigs comprising 15 novel and 3 previously reported viruses. We further investigated the Lentinula edodes negative-stranded RNA virus 1 (LeNSRV1) isolated from a symptomatic sample, whose virion is a filamentous particle with a diameter of ~15 nm and a length of ~1200 nm. RT-PCR analysis detected LeNSRV1 in 10 of the 56 Chinese L. edodes core collection strains and 6 of the 22 monokaryotic strains from the L. edodes strain HNZMD. Genetic variation analysis showed that the sequences encoding the nucleocapsid protein (ORF2) from all the aforementioned LeNSRV1 positive strains are very conservative. The results presented here may enrich our understanding of L. edodes virus diversity and the characteristics of LeNSRV1, and will promote further research on virus-host interaction in L. edodes.

Keywords: Genetic variation; High-throughput sequencing; Lentinula edodes; Mycoviral diversity; Negative-stranded RNA virus; Sexual transmission.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Nucleocapsid Proteins / genetics*
RNA Viruses* / isolation & purification
RNA Viruses* / physiology
RNA, Viral*
Shiitake Mushrooms / virology*

Substances

Nucleocapsid Proteins
RNA, Viral