Genome-wide DNA methylation analysis of breast cancer MCF-7 / Taxol cells with MeDIP-Seq

Ying Shi; Weihua Gong; Xiangrong Gong; Ping Wang; Xin Zhao

doi:10.1371/journal.pone.0241515

Genome-wide DNA methylation analysis of breast cancer MCF-7 / Taxol cells with MeDIP-Seq

PLoS One. 2020 Dec 11;15(12):e0241515. doi: 10.1371/journal.pone.0241515. eCollection 2020.

Authors

Ying Shi¹, Weihua Gong¹, Xiangrong Gong², Ping Wang¹, Xin Zhao³

Affiliations

¹ Clinical Laboratory, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
² Internal Medicine Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
³ Department of Radiology, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Abstract

Breast cancer (BC) is the most frequently diagnosed tumor in women worldwide. Although the combination of surgery and Taxol chemotherapy can achieve a certain therapeutic effect, patients often develop drug-resistance, resulting in a poor prognosis. Therefore, it is significative to seek the molecular mechanism of chemotherapy resistance. Recent studies have found that abnormal epigenetic regulation in breast cells changes the expression of key genes, which can lead to the occurrence, development, and maintenance of cancer, even related to the development of drug-resistance. Therefore, in this study, we performed methylated DNA immunoprecipitation-sequencing (MeDIP-seq) to reveal the difference in methylation between breast cancer drug-resistant cells and sensitive cells. A total of 55076 differentially methylated genes (DMGs) were detected, including 21061 hypermethylated DMGs and 34015 hypomethylated DMGs. Moreover, Gene Ontology (GO) analysis and KEGG pathway analysis reveal the function and pathway of screening genes. These results indicate that DNA methylation may be involved in regulating the occurrence and development of breast cancer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antineoplastic Agents, Phytogenic / pharmacology
Breast Neoplasms / drug therapy*
Breast Neoplasms / genetics*
Breast Neoplasms / metabolism
DNA Methylation*
Drug Resistance, Neoplasm / genetics*
Epigenesis, Genetic
Female
Gene Expression Profiling
Gene Ontology
Gene Regulatory Networks
Humans
Immunoprecipitation
MCF-7 Cells
Paclitaxel / pharmacology*
Sequence Analysis, DNA / methods
rac1 GTP-Binding Protein / metabolism

Substances

Antineoplastic Agents, Phytogenic
RAC1 protein, human
rac1 GTP-Binding Protein
Paclitaxel

Grants and funding

This work is supported by the key scientific research project plan of colleges and universities in Henan Province. The project number is: 19A320055. The project name is: The mechanism of pyroptosis in drug resistance of breast tumor cells.