Production of Vitamin B2 (Riboflavin) by Microorganisms: An Overview

Liudmila A Averianova; Larissa A Balabanova; Oksana M Son; Anna B Podvolotskaya; Liudmila A Tekutyeva

doi:10.3389/fbioe.2020.570828

Production of Vitamin B2 (Riboflavin) by Microorganisms: An Overview

Front Bioeng Biotechnol. 2020 Nov 12:8:570828. doi: 10.3389/fbioe.2020.570828. eCollection 2020.

Authors

Liudmila A Averianova¹, Larissa A Balabanova^{1

2}, Oksana M Son^{1

3}, Anna B Podvolotskaya^{1

3}, Liudmila A Tekutyeva^{1

3}

Affiliations

¹ Department of Bioeconomy and Food Security, School of Economics and Management, Far Eastern Federal University, Vladivostok, Russia.
² Laboratory of Marine Biochemistry, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia.
³ ARNIKA, Territory of PDA Nadezhdinskaya, Primorsky Krai, Russia.

Abstract

Riboflavin is a crucial micronutrient that is a precursor to coenzymes flavin mononucleotide and flavin adenine dinucleotide, and it is required for biochemical reactions in all living cells. For decades, one of the most important applications of riboflavin has been its global use as an animal and human nutritional supplement. Being well-informed of the latest research on riboflavin production via the fermentation process is necessary for the development of new and improved microbial strains using biotechnology and metabolic engineering techniques to increase vitamin B2 yield. In this review, we describe well-known industrial microbial producers, namely, Ashbya gossypii, Bacillus subtilis, and Candida spp. and summarize their biosynthetic pathway optimizations through genetic and metabolic engineering, combined with random chemical mutagenesis and rational medium components to increase riboflavin production.

Keywords: food/feed additive; genetically modified microorganisms; metabolic engineering; riboflavin; vitamin B2.

Publication types

Review