Partial revision of the neustonic genus Scapholeberis Schoedler, 1858 (Crustacea: Cladocera): decoding of the barcoding results

Petr G Garibian; Anna N Neretina; Derek J Taylor; Alexey A Kotov

doi:10.7717/peerj.10410

Partial revision of the neustonic genus Scapholeberis Schoedler, 1858 (Crustacea: Cladocera): decoding of the barcoding results

PeerJ. 2020 Nov 25:8:e10410. doi: 10.7717/peerj.10410. eCollection 2020.

Authors

Petr G Garibian^#¹, Anna N Neretina¹, Derek J Taylor², Alexey A Kotov^#¹

Affiliations

¹ A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, Russia.
² Department of Biological Sciences, State University of New York at Buffalo, Buffalo, NY, USA.

^# Contributed equally.

Abstract

Water fleas (Crustacea: Cladocera) are among the most intensively studied freshwater invertebrates. However, ecologically important daphniids that live on the surface layer (neuston) remain taxonomically confused. Here we attempt to reconcile genetic and morphological information for the neustonic genus Scapholeberis Schoedler, 1858 (Cladocera: Daphniidae) and present the first revision of the Scapholeberis kingii species group. We analyzed new and existing mitochondrial DNA sequences (сytochrome C oxidase subunit I gene region) together with morphology for all but one of the known species of the neustonic daphniids. Morphological comparisons of available populations, belonging to the Scapholeberis kingii species group from several Australian, Asian and African localities, revealed, that they are almost identical according to parthenogenetic females. However, Australian populations can be reliably distinguished from Asian ones based on the morphology of gamogenetic females. Mitochondrial DNA data analyses revealed divergent lineages (>17% for the DNA barcoding COI region) for the three different species (Australia, Asia and Africa). Based on this set of data, we redescribed S. kingii Sars, 1888 from Australia, its terra typica, and described a new species, S. smirnovi sp.nov. from the Russian Far East, Korea and Japan. The status of populations from Ethiopia and the Republic of South Africa remained unclear, because in the African material and the putative type material, we found only parthenogenetic females. Our results provide an integrative revision of the S. kingii species group and improve the taxonomic scaffold used for barcoding and genomics for the remaining species groups in the daphniid genus Scapholeberis.

Keywords: Barcoding; Biogeography; Genetics; Integrative taxonomy; Morphology; New species; Scapholeberis.

Grants and funding

Sampling in South Korea and provisory examination of the specimens from this country are supported by a grant from of the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR201701201). Sampling in Ethiopia was supported by the Joint Ethiopian-Russian Biological Expedition (JERBE). The morphological and genetic study is supported by the Russian Science Foundation (project No. 18-14-00325). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.