Use of natural anti-oxidants in experimental animal models of hepatic ischemia-reperfusion injury

Georgios Kyriakopoulos; Georgia Valsami; Christos Tsalikidis; Michail Pitiakoudis; Alexandra K Tsaroucha

doi:10.1016/j.amsu.2020.11.061

Use of natural anti-oxidants in experimental animal models of hepatic ischemia-reperfusion injury

Ann Med Surg (Lond). 2020 Nov 26:60:592-599. doi: 10.1016/j.amsu.2020.11.061. eCollection 2020 Dec.

Authors

Georgios Kyriakopoulos¹, Georgia Valsami², Christos Tsalikidis^{1

3}, Michail Pitiakoudis^{1

3}, Alexandra K Tsaroucha^{1

4}

Affiliations

¹ Postgraduate Program in Hepatobiliary/Pancreatic Surgery, Faculty of Medicine, Democritus University of Thrace, Alexandroupolis, Greece.
² School of Health Sciences, Department of Pharmacy, National and Kapodistrian University of Athens, Greece.
³ 2Department of Surgery, Faculty of Medicine, Democritus University of Thrace, Alexandroupolis, Greece.
⁴ Laboratory of Experimental Surgery, Faculty of Medicine, Democritus University of Thrace, Alexandroupolis, Greece.

Abstract

Background: Ischemia-reperfusion injury (IRI) remains a clinical challenge in liver surgery, trauma and transplantation, contributing to morbidity and mortality worldwide. Thus, its impact, not only on the liver itself but also on remote tissues, has been studied during the last years. Different natural anti-oxidant substances have been researched in animal models, implementing different times of ischemia, aiming to test new therapeutic interventions.

Objective: A literature review has been conducted with two goals: (1) to identify different natural anti-oxidants studied in experimental models; and (2) to summarize the various times of ischemia employed.

Methods: Scientific papers published in PubMed for the period 2000-2020 were searched and reviewed.

Results: More than 30 natural anti-oxidants have been tested. The time of ischemia ranged from 15 to 90 min with 60 min used most frequently, followed by 45 min. No studies were found with time exceeding 90 min.

Conclusions: A significant number of research has been conducted on the use and protective effect of natural anti-oxidants in experimental animal models. Based on the published papers, 45-60 min seems to be the optimal duration of ischemia.

Keywords: Animal models; Anti-oxidants; Hepatic ischemia-reperfusion (I/R) injury; Ischemia duration; Oxidative stress.

Publication types

Review