Kinetic characteristics of long-term repeated fed-batch (LtRFb) l-lactic acid fermentation by a Bacillus coagulans strain

Abstract

Application of degradable plastics is the most critical solution to plastic pollution. As the precursor of biodegradable plastic PLA (polylactic acid), efficient production of l-lactic acid is vital for the commercial replacement of traditional plastics. Bacillus coagulans H-2, a robust strain, was investigated for effective production of l-lactic acid using long-term repeated fed-batch (LtRFb) fermentation. Kinetic characteristics of l-lactic acid fermentation were analyzed by two models, showing that cell-growth coupled production gradually replaces cell-maintenance coupled production during fermentation. With the LtRFb strategy, l-lactic acid was produced at a high final concentration of 192.7 g/L, on average, and a yield of up to 93.0% during 20 batches of repeated fermentation within 487.5 h. Thus, strain H-2 can be used in the industrial production of l-lactic acid with optimization based on kinetic modeling.

Keywords: Bacillus coagulans; Kinetics; Long‐term repeated fed‐batch; l‐Lactic acid.