Anti-bacterial activity of chitosan loaded plant essential oil against multi drug resistant K. pneumoniae

Feng Zhang; G Ramachandran; Ramzi A Mothana; Omar M Noman; Waleed A Alobaid; G Rajivgandhi; N Manoharan

doi:10.1016/j.sjbs.2020.09.025

Anti-bacterial activity of chitosan loaded plant essential oil against multi drug resistant K. pneumoniae

Saudi J Biol Sci. 2020 Dec;27(12):3449-3455. doi: 10.1016/j.sjbs.2020.09.025. Epub 2020 Sep 18.

Authors

Feng Zhang¹, G Ramachandran², Ramzi A Mothana³, Omar M Noman³, Waleed A Alobaid³, G Rajivgandhi², N Manoharan²

Affiliations

¹ Chest Endoscopy Minimally Invasive Area, Shandong Provincial Chest Hospital, Shandong Province 250013, China.
² Department of Marine Science, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India.
³ Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. BOX 2457, Riyadh 11451, Saudi Arabia.

Abstract

The development of antibiotic resistant in K. pneumoniae is an emerging thread worldwide due to the poor antimicrobial drugs. To overcome this issue, researchers are focused on plant material and their essential oils to fight against multi drug resistant bacteria. In this context, the current study was concentrated in medicinal plant of guva leaves and their essential oils to combat multi drug resistant bacterial infections. The essential oils were successfully screened and confirmed by HRLC-MS analysis. The anti-bacterial ability of the compounds were loaded into the chitosan nanoparticles and proved by FT-IR analysis. In addition, the chitosan loaded essential oils morphology was compared with chitosan alone in SEM analysis and suggested that the material was loaded successfully. Further, the anti-bacterial ability of the chitosan loaded essential oils were primarily confirmed by agar well diffusion method. At the 100 µg/mL of lowest concentration of chitosan loaded essential oils, the multi-drug resistant K. pneumoniae was inhibited with 96% and confirmed by minimum inhibition concentration experiment. Hence, all the experiments were proved that the essential oils were successfully loaded into the chitosan nanoparticles, and it has more anti-bacterial activity against multi-drug resistant K. pneumoniae.

Keywords: Anti-bacterial activity; Chitosan; Essential oils; Medicinal plant; Minimum inhibition concentration; Multi-drug resistant bacteria; Nanomaterials.