β-lactoglobulin (β-Lg) allergy in dietary milk seriously affects the use of high-quality milk protein in infants. In order to solve this problem, the expression of miRNA and Th17 cells in milk β-Lg allergic reaction of children's diet was studied.
Method: female BALB/c mice aged 5-6 weeks were selected as the subjects and randomly divided into blank group and β-Lg sensitized group, with 10 mice in each group. On the 1st, 7th and 14th day, the mice in the β-Lg sensitized group were intraperitoneally injected with allergen (Freund's adjuvant + β-Lg). Mice in the blank group were given the same amount of normal saline. Blood samples were collected from the eyeballs of mice to determine the number of inflammatory cells. The contents of Th17 related cytokines and transcription factors in spleen were detected by RT-PCR.
Results: 1. the number of eosinophils and neutrophils in the β-Lg sensitized group were 15.76/mL and 24.36/mL, respectively, which were significantly higher than those in the blank group (P < 0.05); 2. in the mice of β-Lg sensitized group, the expression of miR-146a and miR-155 was abnormal, the number of Th17 cells was abnormally increased, and the expression levels of IL-17 and RORγt were significantly increased; 3. the abnormal expression of miR-146a and miR-155 in the mice of β-Lg sensitized group was positively correlated with the secretion of Th17 related cytokines, which could be used as one of the biological indexes to evaluate allergic reaction.
Conclusion: the number of Thl7 increased abnormally in dietary milk allergy patients. miRNA gene expression and IL-17 expression could be used as one of the biological indicators to evaluate the allergic reaction of β-Lg.
Keywords: Allergic reaction of β-Lg; Colon tissue; IL-17; RORγt; Splenic lymphocyte.
© 2020 The Authors.