Complete Genome Sequence of Francisella tularensis Live Vaccine Strain NR-28537 (BEI Master Cell Bank)

Robert A Player; Kathleen J Verratti; Sarah L Grady; Linda C Beck; Bruce G Goodwin; Christopher G Earnhart; Shanmuga Sozhamannan

doi:10.1128/MRA.01248-20

Complete Genome Sequence of Francisella tularensis Live Vaccine Strain NR-28537 (BEI Master Cell Bank)

Microbiol Resour Announc. 2020 Dec 10;9(50):e01248-20. doi: 10.1128/MRA.01248-20.

Authors

Robert A Player¹, Kathleen J Verratti¹, Sarah L Grady¹, Linda C Beck², Bruce G Goodwin³, Christopher G Earnhart³, Shanmuga Sozhamannan^{4

5}

Affiliations

¹ Johns Hopkins University Applied Physics Laboratory, Laurel, Maryland, USA.
² Joint Research and Development, Inc., Stafford, Virginia, USA.
³ Joint Program Executive Office, Joint Project Lead, Chemical, Biological, Radiological, and Nuclear Defense, Enabling Biotechnologies, Frederick, Maryland, USA.
⁴ Defense Biological Product Assurance Office, Chemical, Biological, Radiological, and Nuclear Defense, Enabling Biotechnologies, Frederick, Maryland, USA shanmuga.sozhamannan.ctr@mail.mil.
⁵ Logistics Management Institute, Tysons, Virginia, USA.

Abstract

The genome of Francisella tularensis live vaccine strain NR-28537 was sequenced by a hybrid approach utilizing an Oxford Nanopore Technologies R9 flow cell and an Illumina MiSeq platform. De novo assembly of the resulting long and short reads produced a single-contig whole-genome sequence.