Vegetable wastes represent an inexpensive and sustainable source of valuable bioproducts for several applications. Natural micro-porous and fibrous materials can be obtained from a very cheap and abundant cellulosic bio-waste. Here we demonstrated that vegetable waste derivatives can be suitable as scaffolds for biosensors and 3D cell growth. Many studies have been addressed to fabricate biocompatible 3D scaffolds for mammalian stem cells cultures and develop novel systems able to reproduce the complexity of the in vivo microenvironment. Many of these products are proprietary, expensive or require chemical synthesis. The recycling and revaluation of vegetable derived tissues to fabricate scaffolds for analytical biosensors 3D stem cell cultures platforms may represent a very low-cost approach for toxicological and environmental analyses. In this approach, potential applications of vegetable-derived tissue for biosensing and 3D stem cell cultures were investigated. Micro-structured scaffolds from stalk of broccoli, named BrcS, were either functionalized for production of enzymatic 3D-biosensors or preconditioned to be used them as 3D-scaffolds for human mesenchymal stem cells cultures. The conditions to fabricate 3D-biosensors and scaffolds for cell growth were here optimized studying all analytical parameters and demonstrating the feasibility to combine these two properties for an innovative solution to ennoble vegetable wastes.
Keywords: 3D-stem cell cultures; Biosensor; Cell proliferation assay; Cellulose scaffold; Glucose oxidase; MSCs.
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