A novel denitrifying phosphorus removal and partial nitriﬁcation, anammox (DPR-PNA) process for advanced nutrients removal from high-strength wastewater

Xingxing Zhang; Chaochao Wang; Peng Wu; Yunkang Xia; Ya Chen; Wenru Liu; Lezhong Xu; Fangnigbe Faustin

doi:10.1016/j.chemosphere.2020.129165

A novel denitrifying phosphorus removal and partial nitriﬁcation, anammox (DPR-PNA) process for advanced nutrients removal from high-strength wastewater

Chemosphere. 2021 Feb:265:129165. doi: 10.1016/j.chemosphere.2020.129165. Epub 2020 Dec 2.

Authors

Xingxing Zhang¹, Chaochao Wang¹, Peng Wu², Yunkang Xia¹, Ya Chen¹, Wenru Liu³, Lezhong Xu³, Fangnigbe Faustin¹

Affiliations

¹ School of Environmental Science and Engineering, Suzhou University of Science and Technology, No. 1 Kerui Road, Suzhou, 215009, China.
² School of Environmental Science and Engineering, Suzhou University of Science and Technology, No. 1 Kerui Road, Suzhou, 215009, China; National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, No. 1 Kerui Road, Suzhou, 215009, China; Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, No. 1 Kerui Road, Suzhou, 215009, China. Electronic address: wupengniu@126.com.
³ School of Environmental Science and Engineering, Suzhou University of Science and Technology, No. 1 Kerui Road, Suzhou, 215009, China; National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, No. 1 Kerui Road, Suzhou, 215009, China; Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, No. 1 Kerui Road, Suzhou, 215009, China.

PMID: 33302198
DOI: 10.1016/j.chemosphere.2020.129165

Abstract

This study developed a novel DPR-PNA (denitrifying phosphorus removal, partial nitrification and anammox) process for sustaining high-strength wastewater treatment in a modified continuous flow reactor without external carbon source. After 259-days operation, a synchronous highly-efficient total inorganic nitrogen, PO₄^3--P and COD_cr removal efficiencies of 88.5%, 89.5% and 90.1% were obtained, respectively even influent nitrogen loading rate up to 3.2 kg m^-3 d^-1. Batch tests revealed that denitrifying phosphorus accumulating organisms (DPAOs) using NO₃^--N as electron acceptors significantly enriched (74% in total PAOs), which emerged remarkable positive impacts on deep-level nutrient removal as the key limiting factor. Furthermore, the NO₂^--N inhibitory threshold value (∼20.0 mg L^-1) for DPAOs was identified, which demonstrated as an inhibitory component in excessive recycling NO_x^--N. From the molecular biology perspective, Dechloromonas-DPAOs group (18.59%) dominated the excellent dephosphatation performance, while Nitrosomonas-AOB (ammonia oxidizing bacteria) group (16.26%) and Candidatus_Brocadia-AnAOB (anammox bacteria) group (15.12%) were responsible for the desirable nitrogen loss process. Overall, the present work highlighted the novel DPR-PNA process for nutrients removal is a promising alternation for wastewater of high nitrogen but low carbon.

Keywords: Anammox; Biological nutrient removal; Denitrifying phosphorus removal (DPR); Low C/N ratio; Partial nitrification (PN).

MeSH terms

Bioreactors
Denitrification
Nitrification*
Nitrogen
Nutrients
Phosphorus
Sewage
Waste Disposal, Fluid
Wastewater*

Substances

Sewage
Waste Water
Phosphorus
Nitrogen