Generation and characterization of a murine monoclonal antibody specific for the human T1-CD5 molecule

F Genzano; A Funaro; M Alessio; L B De Monte; G Bellone; L Peruzzi; M Zaccolo; F Caligari-Cappino; F Malavasi

doi:10.1177/172460088700200302

Generation and characterization of a murine monoclonal antibody specific for the human T1-CD5 molecule

Int J Biol Markers. 1987 Sep-Dec;2(3):143-50. doi: 10.1177/172460088700200302.

Authors

F Genzano¹, A Funaro, M Alessio, L B De Monte, G Bellone, L Peruzzi, M Zaccolo, F Caligari-Cappino, F Malavasi

Affiliation

¹ Dipartimento di Genetica, Biologia e Chimica Medica, Università di Torino, Italy.

PMID: 3330108
DOI: 10.1177/172460088700200302

Abstract

Murine monoclonal antibodies (MoAbs) have found widespread applications in the characterization of the molecular and functional features of lymphocyte differentiation antigens. The present paper summarizes the results of our work dealing with the production and selection of a murine MoAb recognizing a molecule expressed during the whole differentiative life of T lymphocytes. The MoAb CB01 resulted to be specific for an apparently unique epitope of the T-cell specific membrane glycoprotein T1-CD5.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Monoclonal / analysis
Antibodies, Monoclonal / biosynthesis*
Antibody Specificity*
Antigen-Antibody Reactions
Antigens, Differentiation, T-Lymphocyte / analysis
Antigens, Differentiation, T-Lymphocyte / immunology*
Binding Sites, Antibody
Binding, Competitive
Electrophoresis, Polyacrylamide Gel
Fluorescent Antibody Technique
Humans
Mice
Mice, Inbred BALB C
Precipitin Tests
T-Lymphocytes / immunology*

Substances

Antibodies, Monoclonal
Antigens, Differentiation, T-Lymphocyte