Revealing lipid droplets evolution at nanoscale under proteohormone stimulation by a BODIPY- hexylcarbazole derivative

Xiaolu Liu; Xin Lu; Tong Zhu; Du Wenli; Yang Zhenghui; Hongzhi Cao; Siyou Wang; Yupeng Tian; Zhongping Zhang; Ruilong Zhang; Senio Campos De Souza; Xiaohe Tian

doi:10.1016/j.bios.2020.112871

Revealing lipid droplets evolution at nanoscale under proteohormone stimulation by a BODIPY- hexylcarbazole derivative

Biosens Bioelectron. 2021 Mar 1:175:112871. doi: 10.1016/j.bios.2020.112871. Epub 2020 Dec 2.

Authors

Xiaolu Liu¹, Xin Lu², Tong Zhu¹, Du Wenli², Yang Zhenghui³, Hongzhi Cao¹, Siyou Wang⁴, Yupeng Tian², Zhongping Zhang⁵, Ruilong Zhang², Senio Campos De Souza⁶, Xiaohe Tian⁷

Affiliations

¹ School of Life Science, Anhui University, Hefei, 230601, PR China.
² Department of Chemistry, Key Laboratory of Functional Inorganic Material Chemistry of Anhui Province, Anhui University, Hefei, 230601, PR China.
³ Institutes of Physical Science and Information Technology, Anhui University, Hefei, 230601, PR China.
⁴ School of Life Science, Wuhan University, Wuhan, 430072, PR China.
⁵ Institutes of Physical Science and Information Technology, Anhui University, Hefei, 230601, PR China; CAS Center for Excellence in Nanoscience, Institute of Intelligent Machines, Chinese Academy of Science, Hefei, PR China.
⁶ Department of Chemistry, University College London, London University College London, Gower Street, London, WC1E 6BT, UK.
⁷ School of Life Science, Anhui University, Hefei, 230601, PR China; Department of Chemistry, Key Laboratory of Functional Inorganic Material Chemistry of Anhui Province, Anhui University, Hefei, 230601, PR China; Institutes of Physical Science and Information Technology, Anhui University, Hefei, 230601, PR China; Department of Chemistry, University College London, London University College London, Gower Street, London, WC1E 6BT, UK; Huaxi MR Research Center (HMRRC), Department of Radiology, Functional and molecular imaging Key Laboratory of Sichuan Province, West China Hospital of Sichuan University, Chengdu, 610041, PR China. Electronic address: xiaohe.t@ahu.edu.cn.

PMID: 33298339
DOI: 10.1016/j.bios.2020.112871

Abstract

Lipid droplets (LDs) ultrastructure progression under proteohormone stimulation provide valuable clues for understanding conditions associated with obesity and diabetes. Current available LDs probes either alter intra-LDs environments or lack photo-resistance; thus, this research domain is poorly understood. In this work, a N-B-O type BODIPY- hexylcarbazole derivative named BoCz-Lip was rationally designed while achieving specific LDs live-cell targeting. BoCz-Lip showed minimal impact on cell viability as well as internal LDs' protein, triglyceride and cholesterol level. Along with its good photostability, it can be used to monitor LDs evolution under super-resolution nanoscopy. More importantly, we concluded how proteohormone could influence LDs ultrastructure, offering a better understanding for correlating diseases at the nanoscale.

Keywords: BODIPY- Hexylcarbazole derivative; Lipid droplets ultrastructure; Proteohormone simulation; STED super-Resolution nanoscopy.

MeSH terms

Biosensing Techniques*
Boron Compounds
Lipid Droplets* / metabolism
Lipid Metabolism
Triglycerides

Substances

4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
Boron Compounds
Triglycerides