Toxicological effects of Ag2O and Ag2CO3 doped TiO2 nanoparticles and pure TiO2 particles on zebrafish (Danio rerio)

Abstract

In this study, the toxic effects of silver oxide and silver carbonate doped TiO₂ nanoparticles (Ag₂O-TiO₂ NPs and Ag₂CO₃-TiO₂NPs), TiO₂ nanoparticles (TiO₂ NPs), and bulk TiO₂ on gene expression, lipid peroxidation, genotoxicity, and histological alterations in zebrafish (Danio rerio) was assessed. The physicochemical properties of the synthesized nanoparticles were evaluated by X-ray diffraction (XRD), Scanning Electron Microscope (SEM), diffuse reflectance spectroscopy (DRS), dynamic light scattering (DLS), and Zeta potential analyses. TiO₂NPs after doping with Ag showed shift to higher wavelengths and decrease of band gap energy. Also, remarkable reduction in the size of Ag-doped TiO₂NPs in comparison with the TiO₂ NPs was observed. According to our results, acute toxicity increased in the order of bulk TiO₂ < TiO₂ NPs < Ag₂O-TiO₂NPs < Ag₂CO₃-TiO₂NPs, respectively. Results of sub-lethal experiments after 30 days of exposure, showed higher expression of Gpx, Hsp70, Ucp-2, and Bax genes, and lower expression of Bcl-2 gene in Ag-doped TiO₂NPs than pure TiO₂ particles (TiO₂ NPs and bulk TiO₂) treatments (p < 0.05). However, the mRNA levels of SOD and CAT genes were significantly higher in pure TiO₂ particles than doped TiO₂NPs (p < 0.05). Moreover, levels of malondialdehyde, abnormalities of peripheral blood cells and severity of histological lesions in liver, gill, intestine and kidney tissues were more evident in Ag-dopedTiO₂ NPs than pure TiO₂ particles. It can be concluded that Ag doping of TiO₂ NPs significantly increased their toxicity and resulted in more histological lesions, apoptosis and oxidative stress than pure TiO₂ particles in adult zebrafish.

Keywords: Bulk TiO(2); Genotoxic effect; Histopathology; Metal-doped nanoparticles; TiO(2) NPs.