Bioavailable wine pomace attenuates oxalate-induced type II epithelial mesenchymal transition and preserve the differentiated phenotype of renal MDCK cells

Gisela Gerardi; Cecilia I Casali; Mónica Cavia-Saiz; María D Rivero-Pérez; Cecilia Perazzo; María L González-SanJosé; Pilar Muñiz; María C Fernández Tome

doi:10.1016/j.heliyon.2020.e05396

Bioavailable wine pomace attenuates oxalate-induced type II epithelial mesenchymal transition and preserve the differentiated phenotype of renal MDCK cells

Heliyon. 2020 Nov 18;6(11):e05396. doi: 10.1016/j.heliyon.2020.e05396. eCollection 2020 Nov.

Authors

Gisela Gerardi¹, Cecilia I Casali^{2

3}, Mónica Cavia-Saiz¹, María D Rivero-Pérez¹, Cecilia Perazzo², María L González-SanJosé¹, Pilar Muñiz¹, María C Fernández Tome^{2

3}

Affiliations

¹ Department of Food Biotechnology and Science, Faculty of Sciences, University of Burgos, Plaza Misael Bañuelos, 09001, Burgos, Spain.
² Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Ciencias Biológicas, Cátedra de Biología Celular y Molecular, Buenos Aires, Argentina.
³ Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Química y Fisicoquímica Biológicas Prof. Dr. Alejandro C. Paladini (IQUIFIB)-Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.

Abstract

The functional renal epithelium is composed of differentiated and polarized tubular cells with a strong actin cortex and specialized cell-cell junctions. If, under pathological conditions, these cells have to resist higher kidney osmolarity, they need to activate diverse mechanisms to survive external nephrotoxic agents such as inflammation and oxidative stress. Wine pomace polyphenols exert protective effects on renal cells. In this study, two wine-pomace products and their protective effects upon promotion and preservation of normal cell differentiation and attenuation of oxalate-induced type II epithelial mesenchymal transition (EMT) are evaluated. Treatment with gastrointestinal and colonic bioavailable fractions from red (rWPP) and white (wWPP) wine pomaces, both in the presence and the absence of oxalate, showed similar cell numbers and nuclear size than the non-treated differentiated MDCK cells. Immunofluorescence analysis showed the reduction of morphological changes and the preservation of cellular junctions for the rWPP and wWPP pre-treatment of cells exposed to oxalate injury. Hence, both rWPP and wWPP attenuated oxalate type II EMT in MDCK cells that conserved their epithelial morphology and cellular junctions through the antioxidant activities of grape pomace polyphenols.

Keywords: Antioxidant; Cancer research; Cell biology; Cell differentiation; Cytoskeleton; E-cadherin; EMT; Food science; Natural product; Oxalate; Plant products; Polyphenol; Polyphenols; Renal system; Wine pomace.