Transcriptome dataset of Babesia bovis life stages within vertebrate and invertebrate hosts

Massaro W Ueti; Wendell C Johnson; Lowell S Kappmeyer; David R Herndon; Michelle R Mousel; Kathryn E Reif; Naomi S Taus; Olukemi O Ifeonu; Joana C Silva; Carlos E Suarez; Kelly A Brayton

doi:10.1016/j.dib.2020.106533

Transcriptome dataset of Babesia bovis life stages within vertebrate and invertebrate hosts

Data Brief. 2020 Nov 17:33:106533. doi: 10.1016/j.dib.2020.106533. eCollection 2020 Dec.

Authors

Massaro W Ueti^{1

2

3}, Wendell C Johnson¹, Lowell S Kappmeyer¹, David R Herndon¹, Michelle R Mousel^{1

3}, Kathryn E Reif^{1

2}, Naomi S Taus^{1

2}, Olukemi O Ifeonu⁴, Joana C Silva^{4

5}, Carlos E Suarez^{1

2}, Kelly A Brayton^{2

3}

Affiliations

¹ Animal Disease Research Unit, USDA-ARS, Pullman, WA, United States.
² Program in Vector-borne Disease, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, United States.
³ Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA, United States.
⁴ Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, United States.
⁵ Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, United States.

Abstract

Babesia bovis is a hemoprotozoan parasite of cattle that has a complex life cycle within vertebrate and invertebrate hosts. In the mammalian host, B. bovis undergoes asexual reproduction while in the tick midgut, gametes are induced, fuse, and form zygotes. The zygote infects tick gut epithelial cells and transform into kinetes that are released into the hemolymph and invade other tick tissues such as the ovaries, resulting in transovarial transmission to tick offspring. To compare gene regulation between different B. bovis life stages, we collected parasites infecting bovine erythrocytes and tick hemolymph. Total RNA samples were isolated, and multiplexed libraries sequenced using paired-end 100 cycle reads of a HiSeq 2500. The data was normalized using the TMM method and analysed for significant differential expression using the generalized linear model likelihood ratio test (GLM LRT) in edgeR. To validate our datasets, ten genes were selected using NormFinder. Genes that had no significant fold change between the blood and tick stages in the RNA-Seq datasets were tested by quantitative PCR to determine their suitability as "housekeeping" genes. The normalized RNA-Seq data revealed genes upregulated during infection of the mammalian host or tick vector and six upregulated genes were validated by quantitative PCR. These datasets can help identify useful targets for controlling bovine babesiosis.

Keywords: Babesia; Bovine; Bovine babesiosis; Gene expression; Kinetes; Rhipicephalus microplus.