Optimization of biosurfactant production from Pseudomonas sp. CQ2 and its application for remediation of heavy metal contaminated soil

Wuyang Sun; Baikang Zhu; Fei Yang; Min Dai; Shama Sehar; Changsheng Peng; Imran Ali; Iffat Naz

doi:10.1016/j.chemosphere.2020.129090

Optimization of biosurfactant production from Pseudomonas sp. CQ2 and its application for remediation of heavy metal contaminated soil

Chemosphere. 2021 Feb:265:129090. doi: 10.1016/j.chemosphere.2020.129090. Epub 2020 Nov 30.

Authors

Wuyang Sun¹, Baikang Zhu², Fei Yang³, Min Dai⁴, Shama Sehar⁵, Changsheng Peng⁶, Imran Ali⁷, Iffat Naz⁸

Affiliations

¹ School of Petrochemical Engineering & Environment, Zhejiang Ocean University, Zhoushan 316022, Zhejiang. Electronic address: meilidejiayushu@163.com.
² School of Petrochemical Engineering & Environment, Zhejiang Ocean University, Zhoushan 316022, Zhejiang. Electronic address: zszbk@126.com.
³ College of Environmental Science and Engineering, Ocean University of China, Qingdao, 266100, China. Electronic address: 2413466391@qq.com.
⁴ School of Environmental and Chemical Engineering, Zhaoqing University, Zhaoqing, 526061, China. Electronic address: 1183492590@qq.com.
⁵ Department of Biology, College of Science, University of Bahrain, PO Box 32038, Sakhir, Bahrain. Electronic address: shamaseher@yahoo.com.
⁶ College of Environmental Science and Engineering, Ocean University of China, Qingdao, 266100, China; School of Environmental and Chemical Engineering, Zhaoqing University, Zhaoqing, 526061, China. Electronic address: pcs005@ouc.edu.cn.
⁷ Department of Environmental Science and Engineering, College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen, 518060, China; Key Laboratory of Optoelectronic Devices and Systems, College of Physics and Optoelectronic Engineering, Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen, 518060, China. Electronic address: engrimran56@gmail.com.
⁸ Department of Biology, Deanship of Educational Services, Qassim University, Buraidah, 51452, Saudi Arabia. Electronic address: iffatkhattak@yahoo.com.

PMID: 33293052
DOI: 10.1016/j.chemosphere.2020.129090

Abstract

The present study was conducted to enhance the biosurfactant production yield of Pseudomonas sp. CQ2 isolated from the Chongqing oilfield (China). Besides, the capability of biosurfactant and underlying mechanism for remediation of heavy metal contaminated soil was also investigated. Our results suggested that maximum biosurfactant production (40.7 g/L) was attained at 35 °C by using soybean oil and ammonium nitrate as carbon and nitrogen sources with pH 7, rotational speed of 175 rpm and inoculation ratio of 3%). The removal efficiencies of 78.7, 65.7 and 56.9% for Cd, Cu and Pb respectively were achieved at optimized bioleaching conditions (pH: 11, soil/solution ratio: 30:1 and non-sterilized soil), comparative tests between common chemical surfactants (SDS, Tween-80) and biosurfactants demonstrated the larger removal capacity of biosurfactants. Through SEM-EDX, it was found that the granular material disappeared, the content of Cd, Cu and Pb decreased significantly, and the soil surface became smooth with hole formation after soil washing following bioleaching. ATR-FTIR results showed that the carboxyl functional groups in biosurfactants could chelate heavy metals. These results indicated that biosurfactants from Pseudomonas sp. CQ2 could effectively eliminate Cd, Cu, and Pb from soil.

Keywords: Bioleaching; Biosurfactant; Heavy metals remediation; Pseudomonas sp. CQ2.

MeSH terms

Biodegradation, Environmental
China
Metals, Heavy* / analysis
Pseudomonas
Soil
Soil Pollutants* / analysis
Surface-Active Agents

Substances

Metals, Heavy
Soil
Soil Pollutants
Surface-Active Agents