Time-restricted feeding downregulates cholesterol biosynthesis program via RORγ-mediated chromatin modification in porcine liver organoids

Kexin Zhang; Hao Li; Zimeng Xin; Yanwei Li; Xiaolong Wang; Yun Hu; Haoyu Liu; Demin Cai

doi:10.1186/s40104-020-00511-9

Time-restricted feeding downregulates cholesterol biosynthesis program via RORγ-mediated chromatin modification in porcine liver organoids

J Anim Sci Biotechnol. 2020 Nov 2;11(1):106. doi: 10.1186/s40104-020-00511-9.

Authors

Kexin Zhang¹, Hao Li¹, Zimeng Xin¹, Yanwei Li¹, Xiaolong Wang¹, Yun Hu¹, Haoyu Liu², Demin Cai^{3

4}

Affiliations

¹ College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, PR China.
² College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, PR China. Haoyu.Liu0523@outlook.com.
³ College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, PR China. demincai@yzu.edu.cn.
⁴ Institute of Epigenetics and Epigenomics, Yangzhou University, Yangzhou, 225009, PR China. demincai@yzu.edu.cn.

Abstract

Background: Time-restricted feeding (TRF) is a dieting strategy based on nutrients availability and diurnal rhythm, shown to improve lipid metabolism efficiency. We have demonstrated previously that retinoic acid-related (RAR) orphan receptor (ROR) γ is the primary transcription factor controlling cholesterol (CHO) biosynthesis program of animals. However, the functional role of RORγ in liver physiology of pigs in response to TRF has not been determined, largely due to the lack of functional models and molecular tools. In the present study, we established porcine liver organoids and subjected them to restricted nutrients supply for 10-h during the light portion of the day.

Results: Our results showed that TRF regimen did not alter hepatocyte physiology, including unchanged cell viability, caspase 3/7 enzyme activity and the gene signature of cell proliferation in porcine liver organoids, compared to the control group (P > 0.05). Furthermore, we found that TRF downregulated the hepatic CHO biosynthesis program at both mRNA and protein levels, along with the reduced cellular CHO content in porcine liver organoids (P < 0.05). Using unbiased bioinformatic analysis of a previous ChIP-seq data and ChIP-qPCR validation, we revealed RORγ as the predominant transcription factor that responded to TRF, amongst the 12 targeted nuclear receptors (NRs) (P < 0.05). This was likely through RORγ direct binding to the MVK gene (encoding mevalonate kinase). Finally, we showed that RORγ agonists and overexpression enhanced the enrichment of co-factor p300, histone marks H3K27ac and H3K4me1/2, as well as RNA polymerase II (Pol-II) at the locus of MVK, in TRF-porcine liver organoids, compared to TRF-vector control (P < 0.05).

Conclusions: Our findings demonstrate that TRF triggers the RORγ-mediated chromatin remodeling at the locus of CHO biosynthesis genes in porcine liver organoids and further improves lipid metabolism.

Keywords: Cholesterol biosynthesis program; Histone modification; Pig; Porcine liver organoids; RORγ; Time-restricted feeding.