Isolation and characterization of Wad Medani virus obtained in the tuva Republic of Russia

Vladimir G Dedkov; Anna S Dolgova; Marina V Safonova; Andrei E Samoilov; Oxana A Belova; Ivan S Kholodilov; Alina D Matsvay; Anna S Speranskaya; Kamil Khafizov; Galina G Karganova

doi:10.1016/j.ttbdis.2020.101612

Isolation and characterization of Wad Medani virus obtained in the tuva Republic of Russia

Ticks Tick Borne Dis. 2021 Mar;12(2):101612. doi: 10.1016/j.ttbdis.2020.101612. Epub 2020 Nov 25.

Authors

Affiliations

¹ Saint-Petersburg Pasteur Institute, Federal Service on Consumers' Rights Protection and Human Well-Being Surveillance, Saint-Petersburg, Russia; Martsinovsky Institute of Medical Parasitology, Tropical and Vector Borne Diseases, Sechenov First Moscow State Medical University, Moscow, Russia. Electronic address: vgdedkov@yandex.ru.
² Saint-Petersburg Pasteur Institute, Federal Service on Consumers' Rights Protection and Human Well-Being Surveillance, Saint-Petersburg, Russia.
³ Anti-Plague Center, Federal Service on Consumers' Rights Protection and Human Well-Being Surveillance, Moscow, Russia.
⁴ Saint-Petersburg Pasteur Institute, Federal Service on Consumers' Rights Protection and Human Well-Being Surveillance, Saint-Petersburg, Russia; Central Research Institute for Epidemiology, Federal Service on Consumers' Rights Protection and Human Well-Being Surveillance, Moscow, Russia.
⁵ Chumakov Institute of Poliomyelitis and Viral Encephalitides FSBSI Chumakov FSC R&D IBP RAS, Moscow, Russia.
⁶ FSBI "Center of Strategic Planning" of the Ministry of Health, Moscow, Russia; Moscow Institute of Physics and Technology, National Research University, Dolgoprudny, Russia.
⁷ Central Research Institute for Epidemiology, Federal Service on Consumers' Rights Protection and Human Well-Being Surveillance, Moscow, Russia.
⁸ Central Research Institute for Epidemiology, Federal Service on Consumers' Rights Protection and Human Well-Being Surveillance, Moscow, Russia; Moscow Institute of Physics and Technology, National Research University, Dolgoprudny, Russia.
⁹ Chumakov Institute of Poliomyelitis and Viral Encephalitides FSBSI Chumakov FSC R&D IBP RAS, Moscow, Russia; Institute for Translational Medicine and Biotechnology, Sechenov First Moscow State Medical University, Moscow, Russia.

PMID: 33291056
DOI: 10.1016/j.ttbdis.2020.101612

Abstract

Wad Medani virus (WMV) belongs to the genus Orbivirus and is a poorly studied arbovirus with unclear medical significance. Presently, a limited number of WMV strains are characterized and available in NCBI GenBank, some isolated many years ago. A new WMV strain was isolated in 2012 from Dermacentor nuttalli ticks collected from sheep in the Tuva Republic, Russia, and sequenced using high-throughput methods. Complete coding sequences were obtained revealing signs of multiple intersegment reassortments. These point to a high variability potential in WMV that may lead to the formation of strains with novel properties. These new data on WMV can promote better understanding of: ecological features of its circulation; relationships within the genus Orbivirus; and the medical significance of the virus.

Keywords: High throughput sequencing; Orbiviruses; Russia; Wad Medani virus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Dermacentor / virology*
High-Throughput Nucleotide Sequencing / veterinary
Molecular Conformation
Orbivirus / chemistry
Orbivirus / isolation & purification*
Phylogeny
Sequence Analysis, RNA / veterinary
Sheep / parasitology*
Sheep / virology
Siberia